The PME free-circulating DNA Kit - IPC16 is based on new technology, called: PME – Polymer Mediated Enrichment. The procedure doesn’t start with sample lysis, like common used methods or kits.
Each Reagent Plate is prefilled and ready to use for 8 samples.
The first step is capturing of free-circulating DNA with a special polymer. Subsequently the captured free-circulating DNA is dissolved in a special buffer and then the DNA is extracted automatically by using the InnuPure® C16 / C16 touch.
The extraction process is based on binding of the free-circulating DNA on surface modified magnetic particles. After washing steps the nucleic acid is eluted from the magnetic particles with low RNase-free water and is now ready to use.
The kit contains a Carrier RNA. Addition of Carrier RNA is recommended if extremely low amount of free-circulating DNA is expected. In this case the addition of Carrier RNA can increase the final yield.
Starting material
Serum, plasma, cell culture supernatants or mediums and other cell-free body fluids from 1 ml up to 5 ml
Urine from 5 ml to 10 ml
Enrichment procedure
1 ml protocol / approx: 10 minutes
5 ml protocol / approx: 30 minutes
Extraction time
1 ml protocol / approx: 70 minutes
5 ml protocol / approx: 80 minutes