The innuDETECT Internal Control DNA/RNA Assay is a highly sensitive, selective tool for detecting internal DNA and RNA controls. The assay is based on a TaqMan exonuclease assay that yields qualitative and quantitative information for verifying results. Using HEX as a reporter fluorophore makes the assay suitable for multiplex applications. The innuDETECT Internal Control DNA / RNA Assay is universally compatible with all commercially available real-time rapidPCR thermocyclers and with PCR thermocyclers that use a HEX test channel.

Specifications

• Flexible for use in all commonly used real-time PCR thermocyclers, accommodating both rapidPCR and PCR formats
• Based on the use of TaqMan exonuclease assays
• Highly sensitive and specific for qualitative and quantitative analyses alike

Starting material
Internal DNA and RNA carrier mix controls from nucleic acid purification kits for different starting materials.

Sensitivity
Detection of fluorescence and of IC DNA or IC RNA.

Detection time
rapid qPCR (qTOWER): approx. 50 min.
Standard qPCR (qTOWER 2.0 or TOptical):
approx. 2 h

Order Information
845-ID-0008010 / 10 reactions / 26,25 €
845-ID-0008100 / 100 reactions / 220,50 €

The qualitative and quantitative identification of different microbiological organisms plays a decisive role in monitoring water quality. This applies to drinking water, industrial water and surface water. Regardless of whether it is a matter of determining the total bacterial load, detecting organisms as a key indicator of hygiene or potential contamination - the innuDETECT Water Pathogen Assays offer a range of excellent detection assays based on real-time PCR. Highly specific TaqMan® probes allow sensitive detection of the corresponding target organism from extracted DNA by detection in the FAM channel. The internal controls also included in the assay offer maximum security with regard to the validation of the results obtained. In addition to reliably detecting isolated pathogens such as Clostridium perfringens or Yersinia enterocolitica using the corresponding innuDETECT assays, the differentiation of Legionella spp. and Legionella pneumophila can be determined efficiently in just one PCR reaction. The innuDETECT Bacteria Quantification Assay also provides reproducible semi-quantitative analysis of the general bacterial load.

Specifications

• Highly specific detection of relevant water pathogens within a very short time
• Differentiation of Legionella pneumophila among Legionella spp. in only one multiplex reaction
• Lyophilized master mixes for adequate shipping and storage
• Maximum efficiency - results available in up to 60 min

Detection principle
real-time PCR
Bacterial target 1 (FAM)
Bacterial target 2 (Cy5)¹
Internal Control (HEX)
¹applicable to innuDETECT Legionella Assay

Starting material
Extracted DNA

Detection Time
60 – 90 min

Sensitivity
Assay specific between 1-10 DNA copies/PCR or depending on the background of the negative control

Order information
innuDETECT Bacteria Quantification Assay
845-IDF-0031024 / 24 reactions / 126,00 €
845-IDF-0031096 / 96 reactions / 399,00 €

innuDETECT Yersinia enterocolitica Assay
845-IDF-0032024 / 24 reactions / 126,00 €
845-IDF-0032096 / 96 reactions / 399,00 €

innuDETECT Legionella Assay
845-IDF-0033024 / 24 reactions / 126,00 €
845-IDF-0033096 / 96 reactions / 399,00 €

innuDETECT Clostr perfringens Assay
845-IDF-0034024 / 24 reactions / 126,00 €
845-IDF-0034096 / 96 reactions / 399,00 €

Assurance of optimal food quality and meeting international standards are important challenges in the food industry. This includes identification of undeclared ingredients of animal origin. Reliable, precise and fast approaches such as Real-Time PCR are increasingly important within this area. The innuDETECT Species ID Assays are a novel TaqMan®-based product line enabling a highly sensitive analysis of e.g. pork, beef, donkey, goat, chicken, turkey and fish DNA.The assays use the FAM channel for specific detection of the target DNA and also have an artificial internal control (IC), which can be used both as an extraction and as an amplification control and is detected in the HEX channel. A universal set-up of these kits in combination with uniform PCR protocols allows simultaneous examination of several targets with different innuDETECT Species ID assays.

Specifications

• Verified for application following manual or automated nucleic acid extraction from diverse food matrices
• Lyophilized reagents for easy PCR set up, to minimize errors, for improved efficiency and to save time
• Outstanding sensitivity of 1 pg DNA/PCR reaction
• Fast analysis due to optimized PCR chemistry: assay time 1 h

Starting Material
Total DNA isolated from:
Meat and sausage products
Dairy products
Gummy bears
Ready-made meals

Detection Time
1 h

Sensitivity
1 pg DNA/PCR Reaction

Order information
innuDETECT Goat Assay
845-IDF-0040024 / 24 reactions / 126,00 €
845-IDF-0040096 / 96 reactions / 399,00 €

innuDETECT Sheep Assay
845-IDF-0050024 / 24 reactions / 126,00 €
845-IDF-0050096 / 96 reactions / 399,00 €

innuDETECT Chicken Assay
845-IDF-0060024 / 24 reactions / 126,00 €
845-IDF-0060096 / 96 reactions / 399,00 €

innuDETECT Turkey Assay
845-IDF-0070024 / 24 reactions / 126,00 €
845-IDF-0070096 / 96 reactions / 399,00 €

innuDETECT Donkey Assay
845-IDF-0080024 / 24 reactions / 126,00 €
845-IDF-0080096 / 96 reactions / 399,00 €

innuDETECT Mammal & Bird Assay
845-IDF-0090024 / 24 reactions / 126,00 €
845-IDF-0090096 / 96 reactions / 399,00 €

innuDETECT Fish Assay
845-IDF-0100024 / 24 reactions / 126,00 €
845-IDF-0100096 / 96 reactions / 399,00 €

innuDETECT Cheese Assay
845-IDF-0110024 / 24 reactions / 157,50 €
845-IDF-0110096 / 96 reactions / 504,00 €

innuDETECT Halal Assay
845-IDF-0120024 / 24 reactions / 157,50 €
845-IDF-0120096 / 96 reactions / 504,00 €

innuDETECT Halal Multiplex Assay
845-IDF-0130024 / 24 reactions / 178,50 €
845-IDF-0130096 / 96 reactions / 525,00 €

innuDETECT Horse Assay Assay
845-IDF-0030024 / 24 reactions / 126,00 €
845-IDF-0030096 / 96 reactions / 399,00 €

innuDETECT Pork Assay
845-IDF-0010024 / 24 reactions / 126,00 €
845-IDF-0010096 / 96 reactions / 399,00 €

innuDETECT Beef Assay
845-IDF-0020024 / 24 reactions / 126,00 €
845-IDF-0020096 / 96 reactions / 399,00 €

Are you looking for a fast and sensitive method for the identification of microorganisms in food samples? Thanks to developments in molecular biological techniques, IST Innuscreen‘s solutions minimize the analysis time with minimal personnel effort and reduce the costs incurred while taking into account the quality requirements of today‘s daily laboratory routines. Benefit from the new TaqMan®-based innuDETECT Food Pathogen Assays, which enable specific analysis for a range of pathogens encountered in food control applications. DNA extraction using our novel SmartExtraction technology and subsequent application of IST Innuscreen‘s innuDETECT Food Pathogen Assays, based on lyophilized master mixes, make the daily use easy and secure.The reproducible real-time PCR results are characterized by very good efficiency, detected in the FAM channel. The artificial internal control integrated in the assays can be used as extraction as well as amplification control and is detected in the HEX channel.

Specifications

• Verified for use after manual and automated nucleic acid extraction
• Lyophilized reagents for easy PCR set-up, to minimize errors, for improved efficiency and for shipping at room temperature
• Internal control included
• Fast analysis due to optimized PCR chemistry: assay time 1 h

Detection principle
Real-Time PCR (TaqMan)
Target-DNA (FAM); Internal Control (HEX)

Starting material
Pathogen DNA isolated from pellets following stomacher cultivation

Detection Time
1 h

Sensitivity
5 Copies/PCR reaction

Order information
innuDETECT Listeria monocytogenes Assay
845-IDF-0021024 / 24 reactions / 126,00 €
845-IDF-0021096 / 96 reactions / 399,00 €

innuDETECT Salmonella spp. Assay
845-IDF-0022024 / 24 reactions / 126,00 €
845-IDF-0022096 / 96 reactions / 399,00 €

innuDETECT Salmonella enterica Assay
845-IDF-0023024 / 24 reactions / 126,00 €
845-IDF-0023096 / 96 reactions / 399,00 €

innuDETECT Campylobacter Assay
845-IDF-0024024 / 24 reactions / 126,00 €
845-IDF-0024096 / 96 reactions / 399,00 €

innuDETECT Shiga Toxin 1 Assay
845-IDF-0025024 / 24 reactions / 126,00 €
845-IDF-0025096 / 96 reactions / 399,00 €

innuDETECT Shiga Toxin 2 Assay
845-IDF-0026024 / 24 reactions / 126,00 €
845-IDF-0026096 / 96 reactions / 399,00 €

innuDETECT E. coli O157 Assay
845-IDF-0027024 / 24 reactions / 126,00 €
845-IDF-0027096 / 96 reactions / 399,00 €

innuDETECT E. coli O104 Assay
845-IDF-0028024 / 24 reactions / 126,00 €
845-IDF-0028096 / 96 reactions / 399,00 €

innuDETECT Listeria spp. Assay
845-IDF-0029024 / 24 reactions / 126,00 €
845-IDF-0029096 / 96 reactions / 399,00 €