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Manual Extraction

Manual Extraction - Spin Filter-based
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  • blackPREP Rodent Tail DNA Kit

    The blackPREP Rodent Tail DNA Kit has been specially optimized for isolating DNA from pieces of mouse and rat tails, guaranteeing extremely high yields in a very short period of time. Like the well-proven innuPREP DNA Kits, blackPREP DNA Kits are likewise based on a reliable separation process using spin filter columns. In addition, the extraction process for pieces of rodent tails is complete within 3 hours max. thanks to a highly efficient sample lysis step that eliminates the need for the traditional overnight lysis process.

    Specifications

    Starting material
    Mouse or rat tail pieces
    Maximum mouse tail length = 1.2 cm
    Maximum rat tail length = 0.6 cm

    Average yield
    Mouse tail pieces (1.2 cm): 30 – 40 µg
    Rat tail pieces (0.6 cm): 35 – 45 µg

    Extraction time
    Lysis: Between 1 and 3 hours max.
    Isolation: approx. 9 minutes

    Binding capacity
    Column binding capacity: > 100 µg DNA

    Average purity
    1,8 – 2,0

    Order information
    845-BP-0010010 / 10 reactions / 31,50 €
    845-BP-0010050 / 50 reactions / 120,75 €
    845-BP-0010250 / 250 reactions / 483,00 €

  • blackPREP FFPE DNA Kit

    The blackPREP FFPE DNA Kit has been designed as a very efficient tool for fast isolation of genomic DNA from FFPE samples for subsequent purposes. The kit can be used with different amounts of FFPE tissue samples / sections, up to a maximum of 50 mg. The extraction procedure is based on a new patented chemistry and combines lysis of FFPE tissue samples with subsequent binding of nucleic acids onto the surface of a spin filter membrane. Thereby the optimized procedure renders an extra step of deparaffinization and the usage of toxic solvents (e.g. xyolol or octane) unnecessary. After several washing steps the nucleic acids are eluted from the membrane by using elution buffer. The extraction is optimized to obtain maximum yield for immediate downstream applications and/or storage for future use.

    Specifications

    Starting material
    FFPE (formalin fixed paraffin embedded) tissue samples
    Maximum amount of tissue: 50 mg

    Average yield
    Depends on the type and starting quantity of the FFPE tissue material

    Extraction time
    Approx. 2.5 hours (incl. lysis)

    Binding capacity

    50 μg genomic DNA

    Average purity
    1,7 – 2,2

    Order information
    845-BP-0021010 / 10 reactions / 42,00 €
    845-BP-0021050 / 50 reactions / 152,25 €
    845-BP-0021250 / 250 reactions / 598,00 €

  • blackPREP Tick DNA/RNA Kit

    The blackPREP Tick DNA/RNA Kit allows researchers to simultaneously isolate DNA and RNA from ticks. This is of particular interest when testing ticks for RNA viruses (such as TBE or FSME) in addition to bacterial pathogens. The kit contains application-specific Lysis Tubes, including beads that have been optimized (in terms of their characteristics and quantity) specifically for homogenizing ticks. Following a subsequent lysis process, the DNA is bound to one filter membrane and the RNA is bound to another. The nucleic acids are then washed and eluted into separate reaction vessels. The nucleic acids of tick-borne pathogens can be detected quickly and easily in further applications.

    Specifications

    Starting material
    Ticks

    Average purity
    1,7 – 2,0

    Extraction time
    Less than 1 hour (including lysis)

    Order information
    845-BP-5100010 / 10 reactions / 52,50 €
    845-BP-5100050 / 50 reactions / 225,75 €

  • innuPREP DNA Micro Kit

    The innuPREP DNA Micro Kit is a fast and effective kit for purifying genomic DNA from small quantities of a wide variety of starting materials. These include, among others, paraffin and tissue samples, whole blood or blood sticks and eukaryotic cells. The patented chemistry underlying the purification method combines extremely fast lysis followed by efficient binding of the genomic DNA to the optimized surface of a spin filter. The bound DNA is then washed and eluted. The result is an excellent yield of highly pure DNA, available for all subsequent applications after only 8 minutes.

    Specifications

    Starting material
    Tissue samples or biopsies up to 5 mg
    Paraffin samples (tissue)
    Eukaryotic cells (max. 1 × 10exp6)
    1 – 50 µl of whole blood
    Blood sticks

    Binding capacity
    Column binding capacity: > 100 µg gDNA

    Average purity
    1,7 – 2,0

    Extraction time
    Approx. 8 minutes after lysis

    Average yield
    Depends on the type and quantity of the starting material

    Order information
    845-KS-1011010 / 10 reactions / 31,50 €
    845-KS-1011050 / 50 reactions / 131,25 €

  • innuPREP DNA Mini Kit 2.0

    The innuPREP DNA Mini Kit 2.0 has been designed as a very efficient tool for fast isolation of genomic DNA from a wide range of starting materials like whole blood (fresh or frozen blood; stabilized with EDTA or citrate, from common blood collection systems), tissue, rodent tails, eucaryotic cells, bacterial cell cultures as well as buccal swabs.
    The extraction procedure is based on a new patented chemistry and combines sample lysis with subsequent binding of nucleic acids onto the surface of a Spin Filter membrane. After several washing steps the nucleic acids are eluted from the membrane by using elution buffer. Extraction chemistry and extraction protocol are optimized to get max-imum of yield..

    Specifications

    Starting material

    • Blood (200/400µl)
    • Eucaryotic cell culture (max. 5 × 10exp6)
    • Tissue samples (up to 20 mg)
    • Rodent tail specimens (0.2 – 0,8 cm)
    • Bacterial cell culture (max. 5 × 10exp10)
    • Buccal swabs

    Average yield
    Depends on the type and quantity of the starting material
    Up to 65 µg

    Exraction time
    Approx. 8 minutes after lysis

    Average purity
    1,8 – 2,0

    Binding capacity
    Column binding capacity: > 100 µg gDNA

    Order information
    845-KS-1042010 / 10 reactions / 31,50 €
    845-KS-1042050 / 50 reactions / 136,50 €
    845-KS-1042250 / 250 reactions / 546,00 €

  • innuPREP Forensic Kit

    The innuPREP Forensic Kit is a sophisticated tool optimized specifically for isolating genomic DNA from tiny samples and from highly contaminated forensic specimens. Extraction has been successfully tested for a multitude of starting materials, such as blood, traces of blood, hair, hair roots, beard stubble, finger nails, postage stamps, cigarette butts, chewing gum, traces of sperm, swab samples and finger prints on a variety of surfaces. The novel extraction chemistry also allows for the recovery of severely degraded nucleic acids.

    Specifications

    Starting material

    • Blood and traces of blood
    • Hair, hair roots and beard stubble
    • Finger nails
    • Stamps and envelopes
    • Cigarette butts
    • Chewing gum
    • Swab samples and fingerprints gained from surfaces
    • Traces of sperm
    • Bone meal

    Quality of DNA
    Free of contamination No inhibiting effect on PCRs and other downstream applications

    Binding capacity
    Column binding capacity: > 100 µg gDNA

    Extraction time
    Approx. 15 minutes after lysis

    Average yield
    Depends on the type and quantity of the sample used

    Order Information
    845-KS-1051010 / 10 reactions / 31,50 €
    845-KS-1051050 / 50 reactions / 157,50 €
    845-KS-1051250 / 250 reactions / 630,00 €

  • innuPREP Plant DNA Kit

    The innuPREP Plant DNA Kit has been specially developed for quickly and easily isolating DNA from an extremely wide variety of plant starting materials (such as leaves, stems, roots, flowers, etc.). In addition to efficient sample lysis, the extraction routine also includes a prefiltration step to effectively remove unlysed plant components. The DNA is subsequently bound to a spin filter column using a novel binding buffer, after which it is washed and then eluted into an elution tube. The extracted nucleic acid is then immediately available for a number of downstream applications and can be stored for future applications.

    Specifications

    Starting material

    • Various plant materials (max. 100 mg)
    • Fresh, frozen or dried plant material
    • Average yield

    Depends on the type and starting quantity of the plant material
    Approx. 3 – 25 µg

    Extraction time
    Approx. 30 – 40 minutes

    Binding capacity
    Column binding capacity: > 50 µg DNA

    Average purity
    1,7 – 2,0

    Order information
    845-KS-1060010 / 10 reactions / 36,75 €
    845-KS-1060050 / 50 reactions / 152,25 €
    845-KS-1060250 / 250 reactions / 598,50 €

  • innuPREP Blood DNA Mini Kit

    The new innuPREP Blood DNA Mini Kit is a highly efficient tool for directly isolating DNA from whole blood samples of up to 400 μl. The new optimized kit for extraction of DNA from blood guarantees extremely high-quality DNA that can be used immediately for photometric determinations, PCR or other downstream applications. The innuPREP Blood DNA Mini Kit is very fast and easy to use. Isolation of genomic DNA – free of inhibitors or impurities – can be completed in just 24 minutes.

    Specifications

    Starting material
    Whole blood samples (up to 400 µl)
    Fresh or frozen blood
    Stabilizers: EDTA or citrate
    Average yield

    Depends on sample and the used volume
    Up to 30 µg gDNA

    Extraction time
    Approx. 24 minutes, including lysis

    Binding capacity
    Column binding capacity: > 50 µg gDNA

    Average purity
    1,7 – 2,0

    Order information
    845-KS-1120010 / 10 reactions / 31,50 €
    845-KS-1120050 / 50 reactions / 126,00 €
    845-KS-1120250 / 250 reactions / 493,50 €

  • deltaPREP Blood DNA Mini Kit (MDX)

    CE-IVD Version for diagnostic purposes!

    The new innuPREP Blood DNA Mini Kit is a highly efficient tool for directly isolating DNA from whole blood samples of up to 400 μl. The new optimized kit for extraction of DNA from blood guarantees extremely high-quality DNA that can be used immediately for photometric determinations, PCR or other downstream applications. The innuPREP Blood DNA Mini Kit is very fast and easy to use. Isolation of genomic DNA – free of inhibitors or impurities – can be completed in just 24 minutes.

    Specifications

    Starting material

    • Whole blood samples (up to 400 µl)
    • Fresh or frozen blood
    • Stabilizers: EDTA or citrate
    • Average yield

    Depends on sample and the used volume
    Up to 30 µg gDNA

    Extraction time
    Approx. 24 minutes, including lysis

    Binding capacity
    Column binding capacity: > 50 µg gDNA

    Average purity
    1,7 – 2,0

    Order information
    31-DP-1000010 / 10 reactions / 42,00 €
    31-DP-1000050 / 50 reactions / 152,25 €
    31-DP-1000250 / 250 reactions / 598,50 €

  • innuPREP Blood RNA Kit

    The innuPREP Blood RNA Kit allows users to extract total cellular RNA from fresh whole blood samples, which were stabilized with either EDTA or citrate. The specially optimized lysis buffer makes lysis extremely efficient, effectively deactivating endogenous and exogenous RNases. Using innuPREP Blood RNA Kit allows researchers to isolate highly pure RNA in an extremely short amount of time while completely omitting the highly toxic Beta-mercaptoethanol traditionally used in the extraction process. Upon elution in RNase-free water, the final RNA is ready-to-use and can be used in subsequent applications immediately.

    Specifications

    Starting material
    0.5 – 1 ml whole blood samples
    Fresh blood
    Stabilizers: EDTA or citrate

    Average yield
    Depends on sample Approx. 1 –8 µg

    Extraction time
    Approx. 45 minutes

    Binding capacity
    Column binding capacity: > 20 µg RNA

    Average purity
    1,8 – 2,1

    Order information 845-KS-2010010 / 10 reactions / 42,00 €
    845-KS-2010050 / 50 reactions / 204,75 € 845-KS-2010250 / 250 reactions / 598,50 €

  • innuPREP Micro RNA Kit

    Using the innuPREP Micro RNA Kit allows researchers to isolate small RNA molecules and achieve high yields. The new, optimized binding buffer system makes it possible to achieve high rates of recovery for small RNA molecules such as mRNA, tRNA, rRNA and snRNA. The first step utilizes well-established spin-filter-column-technology to selectively remove genomic DNA; the RNA is then bound, washed and finally removed from the filter membrane using RNase-free water. Users have the flexibility to adjust the elution volume within a range of 30 µl to 80 µl. Also, the extraction chemistry (DC-Technologie) renders the use of highly toxic beta-mercaptoethanol unnecessary for isolating RNA. The innuPREP Bacteria Lysis Booster is also available to further optimize the lysis of bacterial cells, particularly with regard to bacterial strains that are difficult to lyse.

    Specifications

    Starting material

    • Eucaryotic cells (max. 5 × 10exp6)
    • Tissue samples (max. 20 mg)
    • Gram+ and gram– bacteria (max. 1 × 10exp9)
    • Biopsies

    Average yield
    Depends on the type and quantity of the starting material Up to 100 µg RNA High recovery rate for small RNA molecules

    Extraction time
    Approx. 15 – 40 minutes

    Binding capacity
    Column binding capacity: approx. 100 µg RNA

    Average purity
    1,8 – 2,1

    Order information
    845-KS-2030010 / 10 reactions / 47,25 €
    845-KS-2030050 / 50 reactions / 225,75 €
    845-KS-2030250 / 250 reactions / 892,50 €

  • innuPREP RNA Mini Kit 2.0

    The innuPREP RNA Mini Kit 2.0 is the all-rounder for the extraction of total RNA. The specially optimized lysis buffer system guarantees the isolation of intact RNA and sustainably inactivates endogenous and exogenous RNases. Genomic DNA is removed via an optimized precolumn included in the kit. Accordingly, the otherwise necessary DNase I digestion can be completely omitted. Subsequently, the RNA is bound to a second Spin Filter membrane, washed and finally eluted in 30 - 80 μl RNase-free water. Thus, depending on the starting material, the extraction process can be completed in as little as 15 - 40 minutes. Both the protocol procedures and the Spin Filter columns have been optimized compared to the previous version and guarantee improved quality and higher quantity of isolated nucleic acid.

    To further optimize the lysis of bacterial cells, especially with regard to strains that are difficult to lyse, theinnuPREP Bacteria Lysis Booster is also available.

    Specifications

    Starting material
    Eukaryotic cells (max. 5 x 10exp6)
    Tissue samples (max. 20 mg)
    gram+ and gram- bacteria (max. 1 x 10exp9)
    Biospies

    Extraction time
    Approx. 15 - 40 min

    Quality
    1.8 - 2.1 (A260 : A280)

    Binding capacity
    Approx. 100 μg RNA

    Average yield
    Depends on the type and amount of starting material.
    Up to 100 μg RNA

    Average purity
    1,8 - 2,1

    Ordering information
    845-KS-2040010 / 10 reactions / 42,00 €
    845-KS-2040050 / 50 reactions / 204,75 €
    845-KS-2040250 / 250 reactions / 819,00 €

  • innuPREP FFPE total RNA Kit

    The innuPREP FFPE total RNA Kit allows the extraction of total RNA with an excellent recovery of microRNAs from FFPE (formalin-fixed, paraffin-embedded) tissue samples. The kit is suitable for starting amounts of up to 50 mg. Based on a new kind of chemistry the kit enables the isolation of RNA from FFPE samples without a deparaffinization step and thereby eliminates the use of toxic and hazardous components like octane or xylene. After only 1.5 h containing lysis, binding, washing and elution the whole extraction routine is finished. Thus the protocol allows the extraction of RNA with high quality, ideal integrity and excellent formaldehyde- RNA de-crosslinking. The isolated RNA including microRNAs is directly suitable for reverse transcription, amplification reaction and other amplification-based downstream applications.

    Specifications

    Starting Material
    FFPE (formalin-fixed paraffin-embedded) tissue samples
    Maximum amount of tissue: 50 mg

    Extraction Time
    Less than 1.5 hours (incl. lysis)

    Binding Capacity

    50 μg total RNA

    Average Purity
    1,7 – 2,2

    Average Yield
    Depends on the type and starting quantity of the FFPE tissue material

    Order information
    845-KS-2050010 / 10 reactions / 63,00 €
    845-KS-2050050 / 50 reactions / 273,00 €

  • innuPREP Plant RNA Kit

    The chemistry underlying the innuPREP Plant RNA Kit, which has been specifically adapted for isolating plant materials (e.g. leaves, caulis, root, blossom), guarantees highly efficient lysis and effectively deactivates endogenous and exogenous RNases. The innuPREP Plant RNA Kit contains two lysis buffers in order to process as wide a range as possible of different plant materials, particularly plant components with widely different characteristics. Lysis Buffer R is a universal buffer while Lysis Buffer PL has been specifically adapted to extraction from difficult plants such as roses or potatoes. The total extracted RNA is of excellent quality can be used directly in subsequent applications.

    Specifications

    Starting material
    Various types of plant samples (max. 100 mg)
    Fresh or frozen plant material

    Average yield
    Depends on the type and quantity of the sample up to 70 µg

    Extraction time
    Approx. 30 minutes after homogenization

    Binding capacity
    Column binding capacity: approx. 100 µg RNA

    Average purity
    1,8 – 2,1

    Ordering information
    845-KS-2060010 / 10 reactions / 52,50 €
    845-KS-2060050 / 50 reactions / 231,00 €
    845-KS-2060250 / 250 reactions / 924,00 €

  • innuPREP DNA/RNA Mini Kit

    The innuPREP DNA/RNA Mini Kit is IST Innuscreen‘s all-rounder kit. With binding capacities of 50 µg DNA and 100 µg RNA, respectively, both nucleic acids can be isolated from a single sample to produce excellent quality and yields. Eukaryotic cells, gram+ and gram– bacteria and tissue samples can all be used as starting materials. The genomic DNA and total cellular RNA are available for subsequent downstream applications after only 15 to 40 minutes, each in a separate reaction vessel. The DNA is eluted in 100 µl elution buffer, while the RNA is eluted in 30 to 80 µl RNase-free water.

    Specifications

    Starting material
    Eukaryotic cells (max. 5 × 10exp6)
    Tissue samples (max. 20 mg)
    Gram+ and gram– bacteria (max. 1 × 10exp9)

    Average yield
    Depends on the type and quantity of the starting material
    Up to 60 µg RNA; up to 40 µg DNA

    Extraction time
    Approx. 15 – 40 minutes

    Binding capacity
    Approx. 100 µg RNA; > 50 µg gDNA

    Average purity
    RNA: 1,8 – 2,1; DNA: 1,7 – 2,0

    Order information
    845-KS-2080010 / 10 reactions / 47,25 €
    845-KS-2080050 / 50 reactions / 225,75 €
    845-KS-2080250 / 250 reactions / 892,50 €

  • innuPREP Virus TS RNA Kit

    IST Innuscreen has designed a new extraction kit to meet the current enormous increase in demand for extraction kits and the associated global shortage of resources. The innuPREP Virus TS RNA Kit has been designed for the fast manual isolation of RNA (e.g. Sars-CoV-2) from tracheal swabs such as nasopharyngeal and oropharyngeal swabs samples. The procedure combines lysis of starting material with subsequent binding of viral nucleic acids onto the surface of a spin filter membrane. After several washing steps the viral nucleic acids are eluted from the membrane using RNase-free water. Extraction chemistry and extraction protocol are optimized to obtain maximum yield. In addition, the kit contains Carrier RNA. The additional use of internal control DNA and RNA (IC DNA/RNA) or internal standards, as well as positive and negative controls to monitor the purification, amplification, and detection process is recommended.

    Specifications

    Starting material
    Swab samples such as nasopharyngeal and oropharyngeal swabs samples

    Extraction time
    Approx. 25 minutes

    Average yield
    Depending on sample quality/viral load

    Elution volume
    50-200 μl

    Order information
    845-KS-4710250 / 250 reactions / 546 €

  • innuPREP Virus DNA/RNA Kit

    The innuPREP Virus DNA/RNA allows for the simultaneous purification of viral ssDNA or dsDNA and ssRNA from serum, plasma or other cell-free bodily fluids, from tissue, paraffin or swab samples, or from cell cultures. A novel extraction chemistry guarantees isolation of highly pure viral nucleic acids of excellent quality. The use of spin filter membranes maximizes DNA and RNA yields. In addition, different extraction protocols allow for optimal adaptation to the respective starting material. One major advantage of this kit is the time saved by isolating nucleic acids simultaneously, particularly when using starting materials in which the viral contamination is not clear.

    Specifications

    Starting material

    • Serum, plasma, cerebrospinal fluid (200 µl, 400 µl, 600 μl)
    • Cell culture supernatants (200 μl)
    • Swab samples (200 µl)
    • Stool samples (200 μl)

    Quality
    Positive PCR and TaqMan real-time PCR testing results

    Extraction time
    Approx. 25 minutes

    Order information
    845-KS-4800010 / 10 reactions / 47,25 €
    845-KS-4800050 / 50 reactions / 204,75 €
    845-KS-4800250 / 250 reactions / 819,00 €

  • innuPREP PCRpure Kit

    The innuPREP PCRpure Kit provides an extremely fast, simple and highly efficient method for purifying amplification products directly from PCR reaction mixtures, sequencing reaction mixtures and/or for concentrating PCR products. Purification is based on a two-step method and takes only approx. 3 minutes to complete. The need for wash steps is eliminated, thereby reducing the overall process to binding and elution. The process makes it possible to recover amplification products ranging in size from 60 bp to 30 kb with recovery rates of 60 % to 95 % depending on the length of the amplification product. Also, elution can be performed with a very small volume of only 10 µl, which eliminates the need for specialized »mini-elute« Spin Filter columns.

    Specifications

    Starting material
    PCR reaction mixtures (up to 50 µl)

    Fragment lengths

    60 bp – 30 kb

    Extraction time
    Approx. 3 minutes
    Based on a fast, 2-step process

    Binding capacity
    Column binding capacity: > 20 µg

    Average rate of recovery
    Approx. 60 – 95 %, depending fragment length

    Order information
    845-KS-5010010 / 10 reactions / 21,00 €
    845-KS-5010050 / 50 reactions / 63,00 €
    845-KS-5010250 / 250 reactions / 210,00 €

  • innuPREP Plasmid Mini Kit 2.0

    The innuPREP Plasmid Mini Kit 2.0 allows the fast and easy isolation of plasmid DNA from bacterial cultures. To gain an optimal result, protocol steps as well as the chemistry and spin filters were improved in comparison to the precursor products innuPREP Plasmid Mini Kit and innuPREP Plasmid Mini Kit Plus. The process is based on the combination of an alkaline lysis and binding of the plasmid DNA to a filter membrane after precipitation of the chromosomal DNA and bacterial proteins. Bound plasmid DNA is then washed and finally eluted by using a low-molar buffer. The isolated plasmid DNA can be used for a number of sensitive downstream applications, like transfection, cloning, PCR or sequencing. The new procedure also allows for processing of 0.5 ml to 15 ml of bacterial cultures using a table-top centrifuge (after lysis). Due to the new filter membrane up to 80 µg pDNA can be isolated from one sample.

    Specifications

    Average yield
    Depending on the sample and culture volume
    Typical yield from 2 ml (high copy Plasmid): approx. 6 – 20 µg
    Typical yield from 15 ml (high copy Plasmid): approx. 60 – 80 µg

    Starting material
    Bacteria cultures (0.5 to 15 ml)
    Extraction of high copy plasmid DNA/ low copy plasmid DNA, P1 constructs and cosmid DNA

    Average purity
    1,7 – 2,0

    Extraction time
    Approx. 15 min

    Binding capacity
    Approx. 80 µg pDNA

    Order information
    845-KS-5041010 / 10 reactions / 21,00 €
    845-KS-5041050 / 50 reactions / 73,50 €
    845-KS-5041250 / 250 reactions / 278,25 €
    845-KS-5041500 / 500 reactions / 514,50 €

  • innuPREP DOUBLEpure Kit (PCR Reaction and Gel Clean Up)

    The innuPREP DOUBLEpure Kit was designed for efficient extraction of DNA fragments from TAE or TBE agarose gels and utilizes a novel 2-step technology for purifying amplification products from PCR reaction mixtures and sequencing reaction mixtures. The spin filter column has a binding capacity of over 20 µg, allowing for high yields and excellent quality when isolating DNA fragments. Recovery rates amount to 95 % depending on fragment size. Other features of the innuPREP DOUBLEpure Kit include easy handling, flexible choice of elution volume and fast purification protocols.

    Specifications

    Starting material

    • PCR reaction mixes (up to 50 µl)
    • TAE agarose gels (up to 300 mg)
    • TBE agarose gels (up to 300 mg)
    • Sequencing reaction mixture (up to 50 µl)

    Average recovery rates
    PCR-purification: ca. 60 % – 95 %
    Gel extraction: ca. 60 % – 90 %
    Sequencing reaction purification: > 75 %
    depending on fragment length

    Extraction time
    PCR purification: approx. 3 minutes (2-step process)
    Gel extraction: approx. 20 minutes
    Sequencing reaction purification: approx. 2 minutes (2-step process)

    Fragment lengths
    PCR-purification: > 60 bp – 30 kb
    Gel extraction: 100 bp – 30 kb

    Binding capacity
    Column binding capacity: > 20 µg DNA

    Order information
    845-KS-5050010 / 10 reactions / 21,00 €
    845-KS-5050050 / 50 reactions / 73,50 €
    845-KS-5050250 / 250 reactions / 278,25 €

  • innuPREP Stool DNA Kit

    The innuPREP Stool DNA Kit is suitable both for extracting bacterial DNA from stool samples as well as for isolating genomic DNA from shedded intestinal epithelial cells. The extraction process is based on sample lysis followed by a pre-filtration step to remove undissolved sample particles. The sample DNA is then bound to the surface of a spin filter membrane and washed, and the bound DNA is subsequently eluted from the spin filter column.

    Specifications

    Starting material
    200 – 400 mg of solid stool samples
    200 – 400 µl of liquid stool samples
    Of human or animal origin

    Average yield
    Depends on the type and quantity of the starting material

    Extraction time
    Approx. 30 – 45 minutes

    Binding capacity
    Column binding capacity: > 50 µg DNA

    Average purity
    1,7 – 2,0

    Order information
    845-KS-7010010 / 10 reactions / 42,00 €
    845-KS-7010050 / 50 reactions / 183,75€

  • innuPREP cell-free microRNA Kit

    The innuPREP cell-free microRNA Kit has been designed for very fast and efficient isolation of cell-free microRNAs from exosomes and other microvesicles. This kit can be combined with the innuPREP PME Exosome Enrichment Kit, ultracentrifugation, filtration and similar methods. The innuPREP cell-free microRNA Kit can also be used for isolation of microRNA from unprocessed samples, exosome-depleted supernatant obtained after exosome separation by above-mentioned techniques or from samples selected by size exclusion chromatography. The extraction procedure is based on a new kind of chemistry, which combines a fast lysis step with subsequent efficient binding of microRNA to a spin filter surface, followed by washing of the bound microRNA and finally elution of the microRNA. Recovery rate and quality of the eluted microRNA are excellent.

    Specifications

    Starting material
    Exosomes and other MVs isolated with the use of innuPREP PME Exosome Enrichment Kit Unprocessed sample (e.g. serum, plasma, urine, ascites, cell culture supernatant and other bio-fluids) or exosome-depleted supernatant obtained after exosome separation by above-mentioned techniques

    Extraction time
    Approx. 60 minutes

    Orderin formation
    845-KS-8010010 / 10 reactions / 36,75 €
    845-KS-8010050 / 50 reactions / 120,75 €
    845-KS-8010250 / 250 reactions / 409,50 €

  • innuSOLV RNA Reagent

    The innuSOLV RNA Reagent is a solution for efficiently isolating total RNA from various quantities of different starting materials (such as tissue samples, cells, bacterial cells, plants, etc.). The extraction method is based on a single-step, liquid-phase separation that saves a significant amount of time. The innuSOLV RNA Reagent contains a mixture of phenol and guanidine isothiocyanate in a monophasic solution. After adding chloroform and centrifuging, the homogenization product separates into three phases:

    • A reddish, organic phase at the bottom
    • A whitish interphase
    • A colorless liquid phase on top containing the RNA

    The RNA is then precipitated by adding an alcohol. Extraction with the innuSOLV RNA Reagent produces high-quality nucleic acids with no degradation that are suitable for a variety of different applications within just 1 hour of prep time.

    Specifications

    Preperation time
    Approx. 60 minutes

    Starting material
    Tissue samples (100 mg)
    Monolayer cells
    Cell suspensions (of animal or plant origin; yeast or bacterial cells; max. 5 × 10exp6)

    Order infiormation
    845-SB-2090010 / 10 reactions / 21,00 €
    845-SB-2090100 / 100 reactions / 131,25 €

  • innuSPEED Soil DNA Kit

    The innuSPEED Soil DNA Kit is a tool for extracting microbial DNA from soil samples. The extraction combines rapid homogenization of extremely complex starting material with efficient digestion of microbial cell walls in specially developed Lysis Tubes (using a SpeedMill, Retsch mixer mill or other homogenizer). After mechanical disruption followed by a thermal lysis step, the DNA is selectively bound to the surface of a spin filter membrane. A novel wash buffer efficiently removes inhibitors such as humic acids. Finally, DNA is eluted from the membrane using a low-salt buffer.

    Specifications

    Extraction time
    Homogenization and lysis: 30 minutes
    Purification: approx. 16 minutes

    Average yield
    Depends on the type and quantity of microorganisms in the soil
    Up to 30 µg

    Starting material
    Soil samples (100 – 250 mg)

    Binding capacity
    Column binding capacity: > 30 µg DNA

    Average purity
    1,7 – 2,0

    Order information
    845-KS-1580010 / 10 reactions / 52,50 €
    845-KS-1580050 / 50 reactions / 215,25 €

  • innuPREP DNase I Digest Kit

    The innuPREP DNase I Digest Kit is a highly efficient tool for removing DNA from RNA samples that have been contaminated with DNA. Enzymatic DNA digestion can be conveniently integrated into the RNA isolation process. The DNase I is diluted in the buffer provided and then applied directly onto the spin filter column containing the bound nucleic acids. Digestion is followed by additional wash steps before highly pure RNA is eluted. The reagents provided are free of RNase and therefore also contribute to the quality of the RNA. Efficient DNA removal makes the innuPREP DNase I Digest Kit suitable for applications in which even the tiniest amounts of DNA could distort the results. Potential applications include preparing DNA-free RNA, DNase footprinting, Nick translation or degradation of DNA template when transcribing to cDNA.

    Specifications

    Starting material
    RNA samples contaminated with DNA

    Concentration
    DNase I mit 20 KU/µl

    Preperation time
    20 minutes (digestion only)

    Order information
    845-KS-5200010 / 10 reactions / 31,50 €
    845-KS-5200050 / 50 reactions / 115,50 €
    845-KS-5200250 / 250 reactions / 388,50 €

  • innuPREP Plasmid Midi Kit

    The innuPREP Plasmid Midi Kit has been designed for the extraction of plasmid DNA from up to 100 ml of cultured bacterial cells. The kit uses an optimized chemistry with an increased efficiency for low-and high-copy plasmid DNA purification combined with an efficient endotoxin removal and a DNA precipitation procedure. The precipitated plasmid DNA is from excellent quality and therefore highly suited for a lot of downstream applications like transfection, cloning, sequencing, PCR or in vitro transcription.

    Specifications

    Starting material
    Bacteria cultures (up to 100 ml)
    Extraction of high copy plasmid DNA/ low copy plasmid DNA, P1 constructs and cosmid DNA

    Average purity
    1,7 – 2,0

    Extraction time
    Approx. 90 min

    Order information
    845-KS-5043010 / 10 reactions / 125,00€

Manual Extraction - PME-based (Enrichment)
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  • PME free-circulating DNA Extraction Kit

    Circulating cell-free DNA is of special interest as a diagnostic target, but the amount of free-circulating DNA is usually very low and varies among different individuals. Furthermore, these nucleic acids are present as short fragments, typically smaller than 1000 nt, making the efficient extraction process challenging. Because of the high sample volumes, the protocols of commercially available kits are very labor-intensive as well as time consuming and need a lot of reagents. The PME free-circulating DNA Extraction Kit is based on a new, patent-pending technology called PME – Polymer Mediated Enrichment. As first step cell-free DNA in the entire sample is captured by a polymer. Afterwards this complex is collected as a pellet by centrifugation. Subsequently the captured nucleic acid is dissolved in a special buffer thus reducing the sample volume in the following extraction significantly.

    Specifications

    Starting material
    Serum, plasma and urine
    Cell culture supernatants or mediums
    Other cell-free body fluids (except urine)
    From up to 5 ml (plasma) or 10 ml (urine)

    Field of applications
    Tumor and prenatal diagnosis
    Pathological states, including trauma, sepsis, myocardial infarction, stroke, transplantation, diabetes mellitus, and hematologic disorders

    Extraction time
    From 1 ml starting sample: approx. 30 min
    From 5 ml and 10 ml starting sample: approx 1 h

    Order information
    845-IR-0003010 / 10 reactions / 157,50 €
    845-IR-0003050 / 50 reactions / 735,00 €

  • PME Food DNA Kit

    When it comes to food analysis, reliable and highly efficient DNA extraction from respective food samples is the foundation of the generation of valid results. The PME Food DNA Extraction Kit has been specifically developed for the simple and rapid isolation of DNA from liquid as well as solid food samples such as milk, milk powder, juices, wine, various flours, meat, chocolate, bread, and many more. It is based on the innovative technology called PME - Polymer Mediated Enrichment. Free DNA from liquid samples is captured by a polymer after protein digestion. Both the polymer-DNA complex and other intact cells are then pelleted by centrifugation and dissolved in lysis buffer. This workflow guarantees maximum yield of cell-free DNA frequently present in processed food products. In contrast, solid food samples are directly dissolved in lysis buffer before DNA is purified based on spin filters. Thanks to the optimized chemistry of lysis and binding buffer, critical food samples such as chocolate and tomato paste can be processed easily and the presence of inhibitory substances within the eluate is minimized. Thus, DNA purification by the PME Food DNA Extraction Kit offers ideal conditions for the successful implementation of downstream applications such as PCR-based food authenticity testing.

    Specifications

    Starting material
    Liquid and water-soluble food samples such as milk, milk powder, juice, wine, flour (up to 1 mL)
    Solid food samples, processed food, such as meat, sausage, chocolate, bread (up to 100 mg)

    Average yield
    Depending on sample quality/quantity

    Extraction time
    Liquid samples: ca. 2,5 h
    Solid samples: ca. 2 h

    Average purity
    Depending on sample quality, 1.6 – 2.0

    Order information
    845-IR-0008050 / 50 reactions / 210 €

  • PME Food DNA Enrichment Kit

    The PME Food DNA Enrichment Tool is based on a new technology, called: PME – Polymer Mediated Enrichment. /
    The procedure starts with capturing of cells and cell-free DNA from a large amount of liquid sample by a special polymer.
    If necessary, the instant samples have to be dissolved in distillated water and/or proteins have to be digested with Proteinase K. /
    The next step is the centrifugation of captured DNA. Subsequently, the pellet containing DNA can be dissolved directly in a lysis buffer of kit used for DNA isolation.

    Specifications

    Starting material
    Milk, liquid milk products (1ml of e.g. cream, shake)
    Thick milk products (1g of e.g. yoghurt, condensed milk, kefir)
    Dry milk products (0,6g of e.g. baby milk, dry milk)
    Juice, vinegar, wine (1ml)
    Dry instant products without or with low amount of milk (0,6g)

    Preperation time
    Approximately 30-60 minutes

    Order information
    845-IR-0009050 / 50 reactions / 157,50 €

  • PME Exosome Enrichment Kit

    The PME Exosome Enrichment Kit is based on a new technology, called PME – Polymer Mediated Enrichment. Addition of PME to any type of biological fluid (e.g. serum, plasma, urine, ascites, cell culture supernatant, fractions from EVs size separation by size exclusion chromatog-raphy (SEC)), efficiently immobilizes exosomes within the net created by a special kind of polymer. Subsequently, the sample is centrifuged to form a pellet containing PME/exosomes complex. The obtained supernatant contains free proteins of the sample, including microRNA-carrying proteins, like Ago2, HDL or LDL. The exosomes-containing pellet can be resuspended in a dissolving buffer and used directly for downstream applications like microscopy, NanoSight, Western Blot, etc. The PME/exosomes pellet can be also lysed and exosomal microRNAs can be isolated with the use of innuPREP cell-free microRNA Kit. MicroRNAs from the exosome-depleted supernatant, as well as from the starting material can be isolated by the innuPREP cell-free microRNA Kit as well.

    Specifications

    Starting material

    Serum, plasma, urine, ascites, cell culture supernatants or mediums
    SEC fractions and other cell-free biofluids from 0.2 ml up to 10 ml cell culture (max. 5 x 10exp6 cells)

    Preperation time
    Approximately 35-45 minutes /

    Order information
    845-IR-0010050 / 50 reactions / 183,75 €

  • PME Sewage Water Enrichment Tool

    The PME Sewage Water Enrichment Tool is based on a new technology, called: PME – Polymer Mediated Enrichment. The procedure starts with capturing of cells and cell-free DNA from a large amount of liquid sample by a special polymer. The next step is the centrifugation of captured cells and DNA. Subsequently, the pellet can be dissolved directly in a lysis buffer of the kit used for DNA/RNA isolation.

    Specifications

    Starting material
    20 ml Sewage Water

    Preperation time
    Approximately 30-60 minutes

    Order information
    845-IR-0011050 / 50 reactions / 157,50€

Manual Extraction - Magnet particle-based
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  • innuPREP MP Basic Kit A

    The innuPREP MP Basic Kit A was developed for isolating viral/bacterial DNA and/or RNA from various cell-free body fluids. The separation technology is based on a novel extraction chemistry that allows users to simultaneously bind DNA and RNA to the surface of functionalized magnetic particles, thereby combining the steps of first lysing the starting material and then binding the nucleic acids to magnetic beads. These are then washed and the DNA/RNA is eluted in RNase-free water. The routine is extremely easy to carry out, yet universally applicable and highly efficient.Various magnetic racks for 1.5 - 2.0 ml tubes are available for magnetic separation (not included in scope of delivery).

    Specifications

    Starting material
    Serum, plasma, synovial fluids, saliva, other cell-free bodily fluids and supernatants from cell cultures (200 µL each)
    Biopsies (1 – 5 mg)
    Cell cultures (max 5 x 106)
    Nasopharyngeal swabs
    Stool samples (0.05 – 0.1 g)

    Extraction time
    Approx. 20 min. after lysis

    Order information
    845-KS-4900100 / 100 reactions / 147 €

  • innuPREP Sewage Water DNA/RNA Kit

    The innuPREP Sewage Water DNA/RNA Kit has been designed for the isolation of DNA and RNA from sewage water samples. /
    The kit allows the isolation of DNA/RNA from viruses, bacteriophages and free-circulating DNA/RNA (e.g. plasmid associated DNA coding for bacterial resistances) from sewage water sediment as well as from the cleared supernatant after spinning down the sediment. The latest data shows that nucleic acids from this source could be detected in the sed-iment and the supernatant from sewage water samples.
    The kit contains 3 protocols. The first protocol is for the isolation of nu-cleic acids from sewage sediment, the second from the corresponding supernatant and the third protocol allows the simultaneously isolation of nucleic acids from sewage sediment and from the corresponding su-pernatant.
    The kit combines a unique patented technology for enrichment of bio-molecules with a very efficient extraction of DNA and RNA using mag-netic particles.
    The extraction process starts with 20 ml of sewage water sample. After sedimentation of solid material 10 ml of the resulting supernatant and the resulting sediment (appr. 100 – 200 mg) will be used for the ex-traction of DNA and RNA.
    Depending on the respective protocol the supernatant and the sedi-ment alone or both combined could be used for isolating nucleic acids.

    Specifications

    Starting material
    20 ml sewage water

    Extraction time
    Approx. 60 min. including all steps

    Order information
    845-KS-4920050 / 50 reaction / 157,50 €

  • innuPREP MP Basic FFPE RNA Kit

    The innuPREP MP Basic FFPE RNA Kit has been designed as a tool for very fast and efficient isolation of total RNA (including microRNA) from FFPE samples. The extraction procedure is based on a new kind of chemistry, which combines an efficient lysis step with a subsequent effective binding of total RNA on a magnetic particles surface, followed by washing of the bound RNA and final elution of the RNA. The recovery and the quality of isolated RNA are excellent. The new kind of chemistry allows the isolation of RNA from FFPE samples without the deparaffinization step and thereby excludes the use of toxic and hazardous components like octane or xylene.
    The extraction process is finished within less than 2 hours. The isolated RNA is suitable for reverse transcription, amplification reaction and other amplification based further downstream applications.

    Specifications

    Starting material
    FFPE (formalin fixed paraffin embedded) tissue samples

    Preperation time
    Approximately 120 minutes

    Order information
    845-KS-4910050 / 50 reactions / 115,50€

Manual Extraction - SmartExtraction
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  • smart DNA prep (m)

    Using the smart DNA prep (m) kit, genomic DNA can be isolated from various tissue samples, rodent tails, eukaryotic cells, bacteria and yeasts based on the novel SmartExtraction technology. Smart DNA prep (m) allows for manual sample processing with minimal ressources. Lysis, binding, washing and elution are efficiently performed in a simple thermal shaker (e.g. Biometra TS1 ThermoShaker oder Biometra TSC ThermoShaker by Analytik Jena). The included SmartExtraction Tube contains granules mit Smart Modified Surfaces with an enormous binding capacity for DNA. Their magnetic core allows for quick collection using a magnetic rack (not included). The nucleic acids are submitted to minimal shearing forces and are thus of superior quality and very high molecular weight, thus ideally suited for size-sensitive downstream applications such das NanoPore or PacBio sequencing.

    The innuPREP Bacteria Lysis Booster is also available to further optimize the lysis of bacterial cells, particularly with regard to bacterial strains that are difficult to lyse

    Specifications

    No centrifugation – easy extraction using thermal shaker and magnetic rack
    Highly efficient lysis within 1 h up to 3 h max.
    High yield of high-molecular-weight DNA
    Inlcudes SmartExtraction tubes und granules with Smart Modified Surfaces for active binding of DNA
    Enormous binding capacity without clotting

    Starting material
    Tissue samples up to 100 mg
    Mouse tail pieces up to 1 cm
    Rat tail pieces up to 0.5 cm
    Eukaryotic cells up to 5x 10exp6
    Gram- or Gram+ bacteria (5 x 104 up to 2 x 10exp9 cells)
    Yeast cells 1 x 10exp5 up to 5 x 10exp8

    Extraction time
    Lysis: depending on Starting material
    Extraction: approx. 35 min

    Quality
    1.7 – 2.0

    Average yield
    Depends on sample and used volume

    Order information
    845-KS-8000010 / 10 reactions / 36,75 €
    845-KS-8000050 / 50 reactions / 152,25 €

  • smart Blood DNA Midi prep (m)

    The smart Blood DNA Midi prep (m) is based on manual application of SmartExtraction technology, which allows binding of genomic DNA to the unique Smart Modified Surfaces. Up to 10 ml whole can be prepared. The required lab equipment is minimal and just limited to a thermal shaker, like the Biometra TS1 ThermoShaker or Biometra TSC ThermoShaker and a magnet rack (not included in the scope of delivery). In addition to the 3-step-lysis consisting of lysis of erythrocytes, collection and digest of DNA carrying cells, DNA is efficiently bound, washed and eluted. The routine reduces the shearing power to the nucleic acid to an absolute minimum. The isolated DNA has a high molecular weight up to 500 kbp and shows best quality. Direct downstream applications are immediately possible.

    Specifications

    Easiest handling thanks to SmartExtraction-Technology
    Low need of resources: DNA isolation using a thermal shaker (e.g. Biometra TS1 ThermoShaker or Biometra TSC ThermoShaker)
    Minimal shearing power for purification of high molecular weight DNA up to 500 kbp
    Unique Smart Modified Surfaces for optimal binding capacity and high yields
    Innovative 3-step-lysis consisting of lysis of erythrocytes, collection and digestion of DNA carrying cells

    Starting material
    Whole blood samples of 0.5 to 10 ml
    Fresh or frozen blood
    Stabilizers: EDTA, citrate or heparin

    Extraction time
    Lysis: depending on Starting material
    Extraction: approx. 35 min

    Quality
    1.7 – 2.0

    Average yield
    Depends on sample and used volume
    0.5 ml whole blood: approx. 5 – 15 μg gDNA
    1.0 ml whole blood: approx. 15 – 30 μg gDNA
    2.0 ml whole blood: approx. 40 – 70 μg gDNA
    3.0 ml whole blood: approx. 50 – 90 μg gDNA
    5.0 ml whole blood: approx. 90 – 160 μg gDNA
    10.0 ml whole blood: approx. 160 – 320 μg gDNA

    Order information
    845-KS-8100010 / 10 reactions / 47,25 €
    845-KS-8100050 / 50 reactions / 194,25 €

  • innuPREP SE HMW DNA Kit (m)

    The innuPREP SE HMW DNA Kit (m) kit has been designed for the manual isolation of high molecular weight (HMW) genomic DNA (200 kb – 500+ kb) from tissue samples, eukaryotic cells, rodent tails, bacteria and yeasts based on a patented technology. The procedure starts with the lysis of the starting material. After lysis the sample is transferred into a SmartExtraction Tube (SE Tube). The SE Tube contains a material with unique Smart Modified Surfaces which adsorbs the genomic DNA. After washing steps, the nucleic acid is dissolved from the surface of the modified material and is now ready to use for downstream applications. The whole extraction process is simple to handle. The unique extraction chemistry in combination with Smart Modified Surfaces is optimized to get a maximum of yield and quality. The nucleic acids are submitted to minimal shearing forces and are thus of superior quality and very high molecular weight, thus ideally suited for size-sensitive downstream applications such das NanoPore or PacBio sequencing.

    The innuPREP Bacteria Lysis Booster is also available to further optimize the lysis of bacterial cells, particularly with regard to bacterial strains that are difficult to lyse.

    Specifications

    No centrifugation – easy extraction using thermal shaker and magnetic rack
    Highly efficient lysis within 1 h up to 1.5 h max.
    High yield of ultra high-molecular-weight DNA
    Inlcudes SmartExtraction tubes und granules with Smart Modified Surfaces for active binding of DNA
    Enormous binding capacity without clotting

    Starting material
    Tissue samples (up to 100 mg)
    Mouse tail pieces (up to 1 cm)
    Rat tail pieces (up to 0.5 cm)
    Eukaryotic cells (up to 10exp7 cells)
    Gram- or Gram+ bacteria (10exp5 up to 2 x 10exp9 cells)
    Yeast cells (10exp5 up to10exp9)

    Extraction Time
    Lysis: depending on starting material
    Extraction: approx. 60 min

    Quality
    1.7 – 2.0

    Average Yield
    Depends on sample and used volume

    Order information
    845-KS-8001010 / 10 reactions / 80,00€
    845-KS-8001050 / 50 reactions / 350,00€

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