IST Innuscreen‘s innuTaq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E.coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5’ → 3’ DNA polymerase activity. The extreme thermostability of the polymerase allows incubation at high temperatures (95 ° C). It is recommended for use in routine PCR reactions.
Specifications
Concentration
5 U/µl
(One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C.)
PCR Buffer
10× PCR Buffer with KCl; 100 mM Tris-HCl (pH 8.8 at 25 °C); 500 mM KCl; 0.8 % Nonidet P40
10× PCR Buffer with (NH4)2SO4; 750 mM Tris-HCl (pH 8.8 at 25 °C); 200 mM (NH4)2SO4; 0.1 % Tween 20
Enzyme storage buffer
20 mM Tris-HCl pH 8.0; 100 mM KCl; 1 mM DTT; 0.1 mM EDTA; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % Glycerol
PS-Mg2+ solution
MgCl2 stock solution, 25 mM