The innuSOLV RNA Reagent is a solution for efficiently isolating total RNA from various quantities of different starting materials (such as tissue samples, cells, bacterial cells, plants, etc.).
The extraction method is based on a single-step, liquid-phase separation that saves a significant amount of time. The innuSOLV RNA Reagent contains a mixture of phenol and guanidine isothiocyanate in a monophasic solution. After adding chloroform and centrifuging, the homogenization product separates into three phases:
- A reddish, organic phase at the bottom
- A whitish interphase
- A colorless liquid phase on top containing the RNA
The RNA is then precipitated by adding an alcohol. Extraction with the innuSOLV RNA Reagent produces high-quality nucleic acids with no degradation that are suitable for a variety of different applications within just 1 hour of prep time.
Preperation time
Approx. 60 minutes
Starting material
Tissue samples (100 mg)
Monolayer cells
Cell suspensions (of animal or plant origin; yeast or bacterial cells; max. 5 × 10exp6)