The innuPREP TCT Beer Bacteria DNA Kit uses a specially developed technique to concentrate and isolate bacterial DNA from large volume of beer samples, as well as from turbid materials and bacteria shaking cultures. There are individual isolation protocols for 100 ml beer and up to 10 ml bacteria shaking cultures.
The kit is based on a novel and patent-pending technology that allows biomolecules (cells, bacteria, viruses, bacteriophages, algae, free nucleic acids, proteins) contained in liquid samples to be concentrated and then made available for various other analysis methods. The bacterial DNA is then isolated using a specially optimized kit.
The kit is easy to handle and is divided into the following steps:
1. Target Concentration from beer samples (TCT based on native beer sample or cultivation).
2. Centrifugation of concentrated beer sample or bacterial shake culture.
3. Homogenization of bacteria sample pellet.
4. DNA extraction out of the homogenized bacteria cell pellet.
Sample type/Starting material
Beer (100 ml)
Shake Culture (up to 10 ml)
Extraction Time
The amount of TCT Beads that are used depends on the desired concentration factor (according to the desired target volume) and the time in which the concentration step is to take place
Extract
DNA