innuPREP SE Blood&Eukaryotic Cells UHMW DNA Kit - KFFLX

For blood samples the procedure starts with the lysis of erythrocytes and the subsequent pelleting of the PBMC’s. After addition of 1 x PBS, the cells are resuspended and transferred into the Deep Well Plate. For cultivated eukaryotic cells no preliminary steps are necessary. The extraction process is based on adsorption of the genomic DNA on so called Smart Modified Surfaces and it needs no magnetic particles for DNA binding. That means, the DNA binds directly on the surface of the modified KingFisher Flex Tip Combs. After washing, the genomic DNA is eluted from the Smart Modified Surfaces and is ready for use for subsequent downstream applications.
The whole extraction process just needs simple mixing up and down of the modified Tip Combs. The process is very fast and gives no limitation regarding the binding capacity. So, the kit is optimized to get a maximum of yield and quality. The nucleic acids are submitted to minimal shearing forces and are thus of superior quality and very high molecular weight, thus ideally suited for size-sensitive downstream applications such das NanoPore or PacBio sequencing.

Automated high-throughput application: Purification of up to 96 samples using the KingFisher FLEX (Thermo Fisher Scientific)
High yield of ultra high molecular weight DNA
Enormous binding capacity without clotting