The innuPREP PME cfDNA Kit - PP Mini is based on a new technology, called: PME – Polymer Mediated Enrichment. The procedure does not start with sample lysis, like commonly used methods or kits. The first step is capturing of free-circulating DNA with a special polymer. Subsequently the captured free-circulating DNA is dissolved in a special lysis buffer and then the DNA is extracted.
There are 2 different capturing variants possible with this kit.
1. PME – cfDNA capture followed by centrifugation
2. PME – cfDNA capture and simultaneous binding to magnetic beads - no centrifugation
The kit combines a unique patented technology for enrichment of biomolecules with a very efficient extraction of DNA using magnetic particles.
Starting material
urine (10 ml)
plasma (5 ml)
Low Throughput Device
PurePrep Mini
Extract
DNA
Reactions
16 or 96 (PP Mini)
Extraction time
90 min (including all steps)
Average yield
Depending on sample quality/quantity