The innuPREP PCRpure Kit provides an extremely fast, simple and highly efficient method for purifying amplification products directly from PCR reaction mixtures, sequencing reaction mixtures and/or for concentrating PCR products. Purification is based on a two-step method and takes only approx. 3 minutes to complete.
The need for wash steps is eliminated, thereby reducing the overall process to binding and elution. The process makes it possible to recover amplification products ranging in size from 60 bp to 30 kb with recovery rates between 60 % to 95 % depending on the length of the amplification product. Also, elution can be performed with a very small volume of only 10 µl, which eliminates the need for specialized Mini-elute Spin Filter columns.
Starting material
PCR reaction mixtures (up to 50 µl)
Fragment lengths
>60 bp - 30 kb
Extraction time
Approx. 3 minutes
Based on a fast, 2-step process
Binding capacity
Column binding capacity: > 20 µg
Average rate of recovery
Approx. 60 – 95 %, depending fragment length