The innuPREP MP Basic FFPE RNA Kit has been designed as a tool for very fast and efficient isolation of total RNA (including microRNA) from FFPE samples. The extraction procedure is based on a new kind of chemistry, which combines an efficient lysis step with a subsequent effective binding of total RNA on a magnetic particles surface, followed by washing of the bound RNA and final elution of the RNA.
The recovery and the quality of isolated RNA are excellent. The new kind of chemistry allows the isolation of RNA from FFPE samples without the deparaffinization step and thereby excludes the use of toxic and hazardous components like octane or xylene.
The extraction process is finished within less than 2 hours. The isolated RNA is suitable for reverse transcription, amplification reaction and other amplification based downstream applications.
Starting material
FFPE (formalin fixed paraffin embedded) tissue samples
Preperation time
Approximately 120 minutes