innuPREP FFPE total RNA Kit

The innuPREP FFPE total RNA Kit allows the extraction of total RNA with an excellent recovery of microRNAs from FFPE (formalin-fixed, paraffin-embedded) tissue samples.

845-KS-2050010

The kit is suitable for starting amounts of up to 50 mg. Based on a new kind of chemistry the kit enables the isolation of RNA from FFPE samples without a deparaffinization step and thereby eliminates the use of toxic and hazardous components like octane or xylene. Lysis, binding, washing and elution is finished within 1.5 h. Thus the protocol allows the extraction of RNA with high quality, ideal integrity and excellent formaldehyde- RNA de-crosslinking. The isolated RNA including microRNAs is directly suitable for reverse transcription, amplification reaction and other amplification-based downstream applications.

Sample type/Starting material
FFPE (formalin-fixed, paraffin embedded) tissue
Specifications

Starting Material
FFPE (formalin-fixed paraffin-embedded) tissue samples
Maximum amount of tissue: 50 mg

Extraction Time
Less than 1.5 hours (incl. lysis)

Binding Capacity
>50 μg total RNA

Average Purity
1,7 – 2,2

Average Yield
Depends on the type and starting quantity of the FFPE tissue material

Extract
RNA
Reactions
10 / 50 / 100 / 250 (either or)
Order information
845-KS-2050010 / 10 reactions / € 63.00
845-KS-2050050 / 50 reactions / € 273.00