The innuPREP DNA/RNA Mini Kit is IST Innuscreen‘s all-rounder kit. With binding capacities of 50 µg DNA and 100 µg RNA, respectively, both nucleic acids can be isolated from a single sample to produce excellent quality and yields.
Eukaryotic cells, gram+ and gram- bacteria and tissue samples can all be used as starting materials. The genomic DNA and total cellular RNA are available for subsequent downstream applications after only 15 to 40 minutes, each in a separate reaction vessel. The DNA is eluted in 100 µl elution buffer, while the RNA is eluted in 30 to 80 µl RNase-free water.
Starting material
Eukaryotic cells (max. 5 × 10exp6)
Tissue samples (max. 20 mg)
Gram+ and gram- bacteria (max. 1 × 10exp9)
Average yield
Depends on the type and quantity of the starting material
Up to 60 µg RNA; up to 40 µg DNA
Extraction time
Approx. 15 – 40 minutes
Binding capacity
Approx. 100 µg RNA; > 50 µg gDNA
Average purity
RNA: 1,8 – 2,1; DNA: 1,7 – 2,0