The innuPREP DNA Mini Kit 2.0 has been designed as a very efficient tool for fast isolation of genomic DNA from a wide range of starting materials like whole blood (fresh or frozen blood; stabilized with EDTA or citrate, from common blood collection systems), tissue, rodent tails, eucaryotic cells, bacterial cell cultures as well as buccal swabs.
The extraction procedure is based on a new patented chemistry and combines sample lysis with subsequent binding of nucleic acids onto the surface of a Spin Filter membrane. After several washing steps the nucleic acids are eluted from the membrane by using elution buffer. Extraction chemistry and extraction protocol are optimized to get maximum of yield..
Starting material
- Blood (200/400µl)
- Eukaryotic cell culture (max. 5 × 10exp6)
- Tissue samples (up to 20 mg)
- Rodent tail specimens (0.2 – 0,8 cm)
- Bacterial cell culture (max. 5 × 10exp10)
- Buccal swabs
Average yield
Depends on the type and quantity of the starting material
Up to 65 µg
Exraction time
Approx. 8 minutes after lysis
Average purity
1,8 – 2,0
Binding capacity
Column binding capacity: > 100 µg gDNA