The innuPREP cell-free microRNA Kit has been designed for very fast and efficient isolation of cell-free microRNAs from exosomes and other microvesicles.
This kit can be combined with the innuPREP PME Exosome Enrichment Kit, ultracentrifugation, filtration and similar methods. The innuPREP cell-free microRNA Kit can also be used for isolation of microRNA from unprocessed samples, exosome-depleted supernatant obtained after exosome separation by above-mentioned techniques or from samples selected by size exclusion chromatography. The extraction procedure is based on a new kind of chemistry, which combines a fast lysis step with subsequent efficient binding of microRNA to a spin filter surface, followed by washing of the bound microRNA and finally elution of the microRNA. Recovery rate and quality of the eluted microRNA are excellent.
Starting material
Exosomes and other MVs isolated with the use of innuPREP PME Exosome Enrichment Kit Unprocessed sample (e.g. serum, plasma, urine, ascites, cell culture supernatant and other bio-fluids) or exosome-depleted supernatant obtained after exosome separation by above-mentioned techniques
Extraction time
Approx. 60 minutes