The innuPREP Bacteria Lysis Booster has been developed for a highly efficient non-mechanical pre-lysis of bacterial cell walls by generating spheroblasts. This new mixture of different enzymes boosts the lysis of all bacteria, in particular hard-to-lyse microorganisms like Streptococcus spp., Lactobacillus spp., Staphylococcus spp., Bacillus spp. and Clostridium spp..
The resulting spheroblasts can be used in all lysis systems for isolation of DNA, RNA. The best results will be obtained by usage of IST Innuscreen‘s kits. In combination with the newly introduced SmartExtraction technology by IST Innuscreen, the obtained DNA is not only of outstanding yield and quality but also of high-molecular-weight and therefore well suited for size-sensitive downstream applications such das NanoPore or PacBio sequencing.

Specifications

Starting material
Up to 2 x 10exp9 of bacteria cells
Gram + or gram - bacteria

Average yield
Depending on amount of starting material and condition of bacterial cells.
The yield of isolated DNA, RNA is affected by the concentration of starting material and by the extraction kit used.

Extraction time
Approx. 30 minutes

Order information
845-KA-1000050 / 50 reactions / 189,00 €

IST Innuscreen‘s innuTaq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E.coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5’ → 3’ DNA polymerase activity. The extreme thermostability of the polymerase allows incubation at high temperatures (95 ° C). It is recommended for use in routine PCR reactions.

Specifications

Concentration
5 U/µl

(One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C.)

PCR Buffer
10× PCR Buffer with KCl; 100 mM Tris-HCl (pH 8.8 at 25 °C); 500 mM KCl; 0.8 % Nonidet P40
10× PCR Buffer with (NH₄)_2SO4; 750 mM Tris-HCl (pH 8.8 at 25 °C); 200 mM (NH₄)_2SO4; 0.1 % Tween 20

Enzyme storage buffer
20 mM Tris-HCl pH 8.0; 100 mM KCl; 1 mM DTT; 0.1 mM EDTA; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % Glycerol

PS-Mg₂+ solution
MgCl₂ stock solution, 25 mM

Order information
845-EZ-1000050 / 50 reactions / 10,50 €
845-EZ-1000500 / 500 reactions / 84,00 €

The innuTaq HOT-A DNA Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation.
The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5‘ → 3‘ direction in the presence of magnesium. It also possesses a 5‘ → 3‘ polymerization-dependent exonuclease replacement activity but lacks a 3‘ → 5‘ exonuclease activity. innuTaq HOT-A DNA Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.

Specifications

Concentration
5 U/µl

(One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C.)

PCR Buffer
10× Hot Start Buffer complete; 200 mM Tris-HCl (pH 8.5 at 25 °C); 500 mM KCl; 15 mM MgCl₂
10× Hot Start Buffer without MgCl ₂ ; 200 mM Tris-HCl (pH 8.5 at 25 °C); 500 mM KCl

Enzyme storage buffer
20 mM Tris-HCl; 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % (v/v) Glycerol; pH 8.0 (25 °C)

PS-Mg₂+ solution
MgCl₂ stock solution 25 mM Store at –20 °C, avoid frequent thawing and freezing.

Order information
845-EZ-3000050 / 50 reactions / 21,00 €
845-EZ-3000500 / 500 reactions / 210,00 €

The innuDRY qRT-PCR Mix Probe is designed for efficient cDNA synthesis and subsequent qPCR in a single tube. It can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. The Mix can be used for the probe-detection technology including TaqMan and Rehybridization Probe System.The freeze-dried formulation of 5x MasterMix allows shipmentand storage without cooling.
Only template, primers and probe need to be added to the reaction and the final volume should be filled up with PCR-grade water.

Specifications

• Stable enzyme and mix for ambient shipment and room-temperature storage
• Best in-class performance for both single and multiplex detection
• Convenient master mix for detection of low-copy pathogen targets
• High specificity and sensitivity across a wide range of sample sources

Concentration
5x Mix

Order information
845-RT-9000100 / 100 reactions / 14700 €
845-RT-9000200 / 200 reactions / 273,00 €

inNucleotide Set is a Set of dATP, dCTP, dGTP and dTTP in a concentration of 100 mM each. The solution is ready to use and the total amount of dNTP in each tube is 25 µmol.

Order information
845-AS-1100025 / 25 µl each dNTP / 12,60 €
845-AS-1100250 / 250 µl each dNTP / 99,75 €

The innuMIX qPCR MasterMix Probe has been developed for fast, highly reproducible, real-time PCR and validated on the most common real-time instruments. The master mix can be readily combined with a wide variety of probe systems, including TaqMan and rehybridization probes. By delivering the perfect combination of the latest chemistry and PCR enhancer developments with a hot-start Taq DNA polymerase, this product allows researchers to achieve highly specific, ultrasensitive, real-time PCR results.It also significantly reduces the time required to prepare the qPCR batch, while making the process radically easy to handle. Users only need to add template, probes and primers to the reaction, then add water (suitable for real-time PCR) up to the final volume. The set-up can readily be carried out at room temperature.

The concentration of MgCl ₂ in the master mix is already ideal, eliminating the need to add any more.

Specifications

• Includes innuTaq Hot-A DNA Polymerase and high-quality dNTPs in a 2x formulation
• Simple, well-established handling combined with superior reproducibility
• Excellent PCR efficiency and slope
• Ideal for most commercially available real-time PCR instruments

Concentration
2x Master Mix

Order information
845-AS-1200015 / 15 reactions / 10,50 €
845-AS-1200100 / 100 reactions / 63,00 €
845-AS-1200200 / 200 reactions / 115,50 €
845-AS-1201000 / 1000 reactions / 556,50 €

The innuMIX qPCR MasterMix SyGreen Sensitive was developed for fast, highly reproducible real-time PCR and validated on the most popular real-time PCR devices for low-ROX and no-ROX. The innuMIX qPCR MasterMix SyGreen uses a proprietary intercalating dye, which does not inhibit PCR. The MasterMix can be used to quantify any DNA template including genomic, cDNA and viral sequences. The proprietary technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity.

Specifications

• Includes highest quality dNTP‘s in 2x Formation, SyGreen an LowROX
• Very high specificity and PCR efficiency
• Non-PCR inhibiting intercalating dye
• The MasterMix is compatible with most real-time PCR instruments - standard and fast cycling conditions
• Perfectly suitable for low copy number targets, G/C rich sequences and targets with an amplification longer than 300 bp

Concentration
2x MasterMix

Order information
845-AS-1310015 / 15 reactions / 21,00 €
845-AS-1310100 / 100 reactions / 210,00 €
845-AS-1310200 / 200 reactions / 399,00 €
845-AS-1310500 / 500 reactions / 924,00 €

The innuMIX qPCR DSGreen Standard is one of the best choice products for fast and highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments with NO-ROX requirements. It contains all reagents required for real-time PCR and is designed to achieve excellent results in reaction efficiency and slope.

Specifications

• Improved specificity of Hot-Start DNA Polymerase
• Includes high quality dNTP‘s in 2x Formation
• Very high specificity and PCR efficiency
• Non-PCR inhibiting intercalating dye
• The MasterMix is compatible with most real-time PCR instruments - standard and fast cycling conditions
• Perfectly suitable for crude samples (e.g.blood samples)

Concentration
2x MasterMix

Order information
845-AS-1320015 / 15 Reaktionen / 10,50 €
845-AS-1320100 / 100 Reaktionen / 73,50 €
845-AS-1320200 / 200 Reaktionen / 136,50 €
845-AS-1320500 / 500 Reaktionen / 325,50 €

The innuDRY qPCR MasterMix Probe is a novel, storage-stable real-time PCR Master Mix for fluorescence detection using different probe technologies (e.g. hydrolysis, FRET or rehybridization probes, and many more). The mix contains all components for a successful real-time PCR. Besides high-quality dNTP’s and a perfect combination of qPCR enhancers, the hot start polymerase avoids unspecific side products selectively. Additionally, the lyophilized 2-times formulation offers high storage stability at +4 °C and an environmentally friendly delivery at room temperature. After dissolving by using the Resuspension Buffer only the primer, probes and template need to be added to the master mix and filled up to the final volume by using molecular-biology-grade water. The established handling makes the daily use easy and secure. Real-time results are highly reproducible and ideal in terms of efficiency and slope.

Specifications

• Ideal for usage with different probe detection technologies (incl. Taqman^® or Rehybridization Probes)
• Environment-friendly delivery at room temperature
• 2-times concentrated for easy and safe handling
• Compatible with most real-time PCR thermal cyclers
• Including high-quality dNTP’s and a Taq DNA Polymerase with Hot Start function
• High storage stability at +4 °C

Concentration
2x MasterMix

Order information
845-AS-1900100 / 100 reactions / 105,00 €
845-AS-1900200 / 200 reactions / 1890,00 €

In the daily routine, simple and safe processes are particularly important, which guarantee the high reproducibility of the final results. In addition, it is important that reagents used are stable and work reliably. The innuDRY Standard PCR MasterMix combines all requirements. The lyophilized mix is long-term storage stable and can be dissolved by the included Resuspension Buffer at any time. After addition of template and specific primers the PCR reaction just needs to be filled up to final volume by using molecular-biology-grade water and is ready-to-use for subsequent amplification. The use of innuDRY Standard PCR MasterMix can be easily combined with most commercially available thermal cyclers. Thereby quality as well as yield of amplified PCR products is excellent.

Specifications

• Easy handling thanks to 2times concentration
• Environmentally beneficial: stable and delivered at room temperature
• Ready-to-use including hot start Taq DNA Polymerase, dNTP’s and optimal buffer conditions
• Ideal results especially for routine PCR applications
• Safe and secure
• Minimizing of necessary hands-on steps

Concentration
2x MasterMix

Order information
845-AS-2100100 / 100 reactions / 68,25 €
845-AS-2100200 / 200 reactions / 126,00 €

IST Innuscreen‘s ready-to-use nucleotide mix provides highest quality of desoxynucleotides. All dNTPs are ultra pure (> 98 %) and quality-checked by a set of PCR, RT-PCR and Klenow reactions. It is supplied as a 50-fold concentrated mix of ultra pure dATP, dCTP, dGTP and dTTP with 12.5 mM each. The total amount of dNTP in each tube is 25 µmol (6.25 µmol of each dNTP).

Specifications

Concentration
12.5 mM pro dNTP

Quality Control
The desoxynucleotide solution has a purity of > 98 % and is functionally tested by real-time amplification of 30 kb DNA fragments.

Packaging Size
2 × 25 µmol Store at –20 °C, avoid frequent thawing and freezing.

Order information
845-AS-9000010 / 10 reactions / 10,50 €
845-AS-9000100 / 100 reactions / 73,50 €

The Proteinase K is one of the most active endopeptidases known. The enzyme is extraordinarily effective against native proteins and can be used for quickly inactivating endogenous RNases and DNases. Proteinase K is particularly suitable for isolating nucleic acids for use in amplification reactions, for isolating native RNA and DNA from tissues and cell lines, for promoting cell lysis by activating a bacterial autolysis factor, and for modifying proteins and/or glycoproteins on cell surfaces (for membrane structure analyses).

Inhibitors: None of the following inactivate the enzyme: metal ions, chelating agents (such as EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.

Activators: Proteinase K activity is stimulated by the presence of denaturing agents (SDS and urea).

Note: SDS can produce a seven-fold increase in Proteinase K activity.

Optimum pH: Proteinase K is stable over a broad pH range (4 to 12.5), and retains its full activity for several hours if incubated at a pH between 6.5 and 9.5.

The enzyme can reduce proteins to free amino acids if a large excess of protein is present and if incubated for long periods of time.

Specifications

Contains no RNases or DNases, and virtually no DNA
Consistent quality and performance
Robust enzyme: stable over a broad pH range
Ideal for a diverse array of applications, such as preparing cell lysates for subsequent nucleic acid isolation

Concentration
20 mg/mL at an activity of 20 U/mg

Quality Control
Proteinase K is lyophilized and purified via chromatography, after which it is tested to ensure that no RNases, DNases and exonucleases are present. These should not be detectable.

Order information
845-CH-0010006 / 6 mg / 8,40 €
845-CH-0010030 / 30 mg / 26,25 €