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Automomated Extraction / InnuPure C16

Automated Extraction with InnuPure C16touch - Magnetic particle based
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  • innuPREP Blood DNA Mini Kit-IPC16

    The innuPREP Blood DNA Mini Kit-IPC16 can be used for isolating genomic DNA from up to 200 μL of whole blood. The samples can be fresh or frozen, and stabilized in either EDTA, heparin or citrate. When performed on the InnuPure C16 touch, extraction is fully automated, yielding highly reproducible results for all 16 samples. The InnuPure C16 touch performs all of the processing for lysis, for subsequent isolation steps and for final elution. In addition to pre-filled, sealed Reagent Strips/Plates that reduce manual pipetting steps, the InnuPure C16 touch also includes an intelligent tip ejection system that minimizes the risk of cross-contamination. The resulting, high-quality DNA is immediately available for additional applications or for storage.

    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Automation for ideal reproducibility
    Improvement of qPCR results: win up to 1.7 Ct values
    Sensitive protocols for optimized yields

    Starting material
    Fresh or frozen whole blood (up to 400 μL)
    Stabilized in EDTA, heparin or citrate

    Average yield
    Depends on the type and quantity of the Starting material
    Whole blood samples: up to 10 μg

    Extraction time
    Lysis: internal
    InnuPure C16 touch protocol: approx. 75 minutes

    Average purity
    1,8–2,0

    Order information
    845-IPP-1116016 / 16 reactions, 8 reaction per plate (prefilled) / 105,00 €
    845-IPP-1116096 / 96 reactions, 8 reaction per plate (prefilled) / 577,50 €
    845-IPP-1116480 / 480 reactions, 8 reaction per plate (prefilled) / 2320,50 €

    845-PPP-1116016 / 16 reactions, 8 reaction per plate (non-filled) / 99,75 €
    845-PPP-1116096 / 96 reactions, 8 reaction per plate (non-filled) / 399,00 €
    845-PPP-1116480 / 480 reactions, 8 reaction per plate (non-filled) / 1554,00 €

    845-IPS-1116016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-1116096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • deltaPREP Blood DNA Mini Kit-IPC16 (MDX)

    CE-IVD Version for diagnostic purposes!

    The deltaPREP Blood DNA Mini Kit-IPC16 can be used for isolating genomic DNA from up to 200 μL of whole blood. The samples can be fresh or frozen, and stabilized in either EDTA, heparin or citrate. When performed on the InnuPure C16 touch, extraction is fully automated, yielding highly reproducible results for all 16 samples. The InnuPure C16 touch performs all of the processing for lysis, for subsequent isolation steps and for final elution. In addition to pre-filled, sealed Reagent Strips/Plates that reduce manual pipetting steps, the InnuPure C16 touch also includes an intelligent tip ejection system that minimizes the risk of cross-contamination. The resulting, high-quality DNA is immediately available for additional applications or for storage.

    Specifications

    Automation for ideal reproducibility
    Improvement of qPCR results: win up to 1.7 Ct values
    Sensitive protocols for optimized yields

    Starting material
    Fresh or frozen whole blood (up to 400 μL)
    Stabilized in EDTA, heparin or citrate

    Average yield
    Depends on the type and quantity of the Starting material
    Whole blood samples: up to 10 μg

    Extraction time
    Lysis: internal
    InnuPure C16 touch protocol: approx. 75 minutes

    Average purity
    1,8–2,0

    Order information
    31-DP-4000016 / 16 reactions, 8 reaction per plate (prefilled) / 131,25 €
    31-DP-4000096 / 96 reactions, 8 reaction per plate (prefilled) / 606,90 €
    31-DP-4000480 / 480 reactions, 8 reaction per plate (prefilled) / 2381,40 €

    31-DP-5000016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    31-DP-5000096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP Plant DNA I & Plant DNA II Kit-IPC16

    Using the innuPREP Plant DNA Kit-IPC16 allows users to isolate highly pure genomic DNA from a variety of plant materials. Following efficient homogenization using a SpeedMill, other homogenizer, or a mortar and liquid nitrogen, the plant material is lysed, and proteins and polysaccharides are effectively removed in a single precipitation step. Once it has been filtered, the lysate is transferred to pre-filled Reagent Strips and/or Plates. Nucleic acid extraction proceeds automatically on the InnuPure C16 touch using a magnetic particle separation process. Because the final eluate is highly pure and free of magnetic particles, it can be used immediately in subsequent applications such as qPCR. The kit has already been successfully tested on leaves and herbs, fruits, wood, needles and seeds.

    The innuPREP Plant DNA II Kit-IPC16 is optimized for the isolation of genomic DNA from plant material with a particularly high phenol content.

    Specifications

    Extracts high-quality plant DNA from up to 16 samples in parallel
    Developed and optimized for use with the InnuPure C16 touch automated system
    Effective removal of inhibiting by-products such as secondary plant metabolites
    Highly reproducible yields of the DNA to be isolated

    Extraction time
    Homogenization:
    Homogenizer: approx. 30 seconds–3 minutes
    Liquid nitrogen: approx. 5–10 minutes
    Lysis: approx. 40–45 minutes
    InnuPure C16 touch protocol: approx. 45 minutes

    Starting material
    Fresh or frozen plant material (up to 100 mg)
    Plant material containing a large proportion of water (up to 100 mg)

    Average yield
    Depends on the type and quantity of the Starting material
    Up to 60 μg

    Average purity
    1,8–2,0

    Order information
    innuPREP Plant DNA I Kit-IPC16
    845-IPP-1516016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-1516096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-1516480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-IPS-1516016 / 16 reactions, 1 reaction per strip / 152,25 €
    845-IPS-1516096 / 96 reactions, 1 Reaktion per strip / 750,75 €

    innuPREP Plant DNA II Kit-IPC16
    845-IPP-1616016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-1616096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-1616480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-IPS-1616016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-1616096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP DNA Kit-IPC16

    The innuPREP DNA Kit-IPC16 is the universal solution for using the InnuPure C16 touch to automate DNA extraction from a wide variety of starting materials. Pre-filled, sealed Reagent Strips and/or Plates make the instrument extremely easy to load which is completed within little time. Using a process based on the magnetic particle separation principle, the nucleic acids are then separated, washed and, finally, eluted.
    In addition, an optimal combination of novel chemistry and ultramodern instrumentation all but eliminates the risk of transferring magnetic particles to the eluate. High quality and excellent yields characterize the resulting DNA, which is immediately available for subsequent applications such as real-time PCR.

    Specifications

    Automated purification of up to 16 samples when used in conjunction with the InnuPure C16 touch
    Based on magnetic particle extraction
    Utilizes pre-filled, sealed Reagent Plates and / or Strips
    Effectively prevents any potential cross-contamination

    Starting material
    Tissue samples of up to 20 mg
    Rodent tails up to 1.0 cm in length
    Eukaryotic cells (max. = 5 x 106)

    Average yield
    Depends on the type and quantity of the Starting material
    Tissues and rodent tails: up to 50 μg
    Eukaryotic cells: up to 25 μg

    Extraction time
    Lysis: 10–75 minutes (external)
    InnuPure C16 touch protcol: 45 minutes

    Average purity
    1,8–2,0

    Order information
    845-IPP-2016016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-2016096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-2016480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-IPS-2016016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-2016096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP Forensic Kit-IPC16

    The innuPREP Forensic DNA Kit-IPC16 is an ideal solution to handle smallest forensic samples in an automized process. Thereby the purification of genomic DNA from up to 16 samples in parallel takes place within InnuPure C16 touch. The kit has already been proven with positive result using a large number of starting materials. Those are blood and traces of blood, hair, hair roots and beard stubble, cigarette butts, chewing gum, traces of sperm, swab samples e. g. from ear, as well as finger prints on a variety of surfaces and DNA on a tooth brush. Based on patented technology and the principle of magnetic particle separation, even highly degraded nucleic acids can be recovered. pre-filled, sealed reagent plastic allows a fast, uncomplicated preparation of the device and isolation of very pure DNA for direct downstream applications.

    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Extraction of high quality DNA for immediate downstream applications
    For usage of InnuPure C16 touch and up to 16 samples in parallel

    Starting material
    Blood and traces of blood
    Hair, hair roots and beard stubble
    Finger nails
    Stamps and envelopes
    Cigarette butts, chewing gum
    Swab samples and fingerprints taken from surfaces, ear swab, tooth brush
    Traces of sperm, bone meal

    Extraction time
    Lysis: approx. 120 minutes (external)
    InnuPure C16 touch protocol: approx. 38 - 47 minutes

    Average yield
    Depends on the sample and the amount used

    Average purity
    1,8 - 2,0

    Order information
    845-IPP-2416016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-2416096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-2416480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-PPP-2416016 / 16 reactions, 8 reaction per plate (non-filled) / 99,75 €
    845-PPP-2416096 / 96 reactions, 8 reaction per plate (non-filled) / 399,00 €
    845-PPP-2416480 / 480 reactions, 8 reaction per plate (non-filled) / 1554,00 €

    845-IPS-2416016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-2416096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP RNA Kit-IPC16

    The innuPREP RNA Kit-IPC16, which has been optimized for the InnuPure C16 touch, makes it possible to automate RNA extraction from a wide variety of tissue samples and cells. Lysis is especially efficient and requires no more than 20 to 30 minutes to complete (depending on the starting material). Genomic DNA is then selectively removed via centrifugation with spin filter columns, a step that eliminates the need for time-consuming enzymatic DNA digestion. Subsequent steps, which include binding and washing of the total RNA, as well as the final elution step, take place in the InnuPure C16 touch and are fully automated. The extraction process is based on separation by magnetic particles, which bind RNA due to the adjusted buffer conditions. pre-filled Reagent Strips or Reagent Plates, prevent any potential contamination. The magnetic particles and extraction protocols have been specially adapted for isolating RNA and therefore guarantee highly pure RNA and excellent yields.

    Specifications

    Optimized for isolating total RNA from a wide variety of tissue samples and cells
    No need for highly toxic ß-mercaptoethanol
    Spin Filter column for selective DNA removal reduces time required (no DNase I digestion)
    Greatly reduced contamination risk
    Kit contains all consumables and pre-filled reagents
    Based on magnetic particle separation in the InnuPure C16 touch

    Starting material
    Eukaryotic cells (max. 5 x 10exp6)
    Various tissue samples (max. 20 mg)
    Tissue samples with a high RNA content (e.g. spleen samples, pan-creatic samples, lymph nodes, max. 5 mg)

    Extraction time
    Lysis: Cells approx. 10 to 20 minutes
    Tissue depending on type of tissue and homogenization
    InnuPure C16 touch protocol: approx. 54 minutes

    Average yield
    Depending on type and amount of starting material used
    Eukaryotic cells (e.g. NIH 3T3 or HEK 293) up to 20 μg
    Tissue samples (e.g. mouse spleen) up to 50 μg

    Average purity
    1,8 – 2,1

    Order information
    845-IPP-4116016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-4116096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-4116480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-IPS-4116016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-4116096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP Bacteria DNA Kit-IPC16

    Using the innuPREP Bacteria DNA Kit-IPC16 allows researchers to isolate bacterial DNA both from bacterial cultures (gram+ and gram-) and from tissue samples. The corresponding extraction routine takes place on the InnuPure C16 touch automation system. Following external lysis, the sample is transferred to pre-filled, sealed Reagent Strips/Plates. The subsequent processes of binding the nucleic acid to magnetic particles, washing and final elution are fully automated. The option of initially lysing the cell walls with lysozyme, especially if working with gram+ bacteria, is recommended. Yields and purity of the isolated bacterial DNA are excellent.
    The innuPREP Bacteria Lysis Booster is also available to further optimize the lysis of bacterial cells, particularly with regard to bacterial strains that are difficult to lyse.

    Specifications

    Product Details
    Suitable for tissue samples and for gram- or gram+ bacterial pellets
    Optional cell-wall lysis using lysozyme
    Fast, simultaneous DNA isolation from up to 16 samples thanks to pre-filled Reagent Strips/Plates

    Starting material

    • Bacterial pellets (gram+ and gram-) after culturing (not more than 1 x 10exp9 cells)

    Extraction time
    Lysis: 30 - 40 minutes
    InnuPureC16 touch protocol: approx. 45 minutes

    Average yield
    Depends on the type and quantity of the Starting material
    Up to 25 μg

    Average purity
    1,8–2,0

    Order information
    845-IPP-5516016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-5516096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-5516480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-IPS-5516016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-5516096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP Food DNA Kit-IPC16

    The innuPREP Food DNA Kit-IPC16 is a simple, safe, automated tool for extracting DNA from a variety of food samples. Thanks to pre-filled, sealed Reagent Strips/Plates, using InnuPure C16 touch eliminates the need for time-consuming prep steps. Once the starting material has been homogenized (using a homogenizer such as the SpeedMill PLUS) and external lysis is complete, the InnuPure C16 touch automated extraction system carries out all of the remaining steps. Based on patented technology and the magnetic particle separation principle, the system utilizes a stringent lysis buffer and optimized binding buffer to achieve large yields of high-quality nucleic acids. The isolated DNA contains virtually no inhibitors and is immediately available for subsequent applications (such as qPCR).

    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Product Details
    Isolation of large quantities of highly pure DNA without transferring magnetic particles
    No discoloration of the DNA eluate
    Includes RT-PCR setup recommendations for each food category

    Starting material
    Categorized food samples:
    Meat and sausage products
    Canned foods (frankfurters and fish)
    Convenience foods
    Baked goods, chips, muesli
    Chocolate
    Flour, baking mixtures, spices
    Ketchup, mustard, sauces, jams, bread spreads
    Dairy products
    Oils and fats
    Up to 200 mg of material

    Extraction time
    Lysis: approx. 60 minutes (external)
    InnuPure C16 touch protocol: approx. 38 or 47 minutes

    Average yield
    Depends on the sample and the amount used

    Average purity
    1,8 - 2,0

    Order information
    845-IPP-5716016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-5716096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-5716480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-PPP-5716016 / 16 reactions, 8 reaction per plate (non-filled) / 99,75 €
    845-PPP-5716096 / 96 reactions, 8 reaction per plate (non-filled) / 399,00 €
    845-PPP-5716480 / 480 reactions, 8 reaction per plate (non-filled) / 1554,00 €

    845-IPS-5716016 / 16 reactions, 1 reaction per strip (prefilled) / 1525,25 €
    845-IPS-5716096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP FFPE DNA Kit-IPC16

    The combination of innuPREP FFPE DNA Kit-IPC16 and InnuPure C16 touch offers a platform for easy, safe, automated DNA extraction from formalin-fixed, paraffin-embedded (FFPE) tissue samples. Because of a novel chemistry, the typically used, extensive process to remove paraffin is completely eliminated. Thus the isolation of genomic DNA is done without the use of toxic solvents, like Xylol or Octane. An additional Proteinase K digestion breaks down proteins within cell lysates and releases the nucleic acids. Subsequent DNA isolation is performed by magnetic particle separation and is based on patented technology using the InnuPure C16 touch. Pre-filled, sealed Reagent Strips and/or Plates can significantly reduce the risk of cross-contamination between samples. The highly pure DNA obtained is then available for subsequent applications such as qPCR.

    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Without the need for typically used toxic solvents such as Xylol or Octane
    High-quality DNA from up to 16 samples by InnuPure C16 touch

    Starting material
    FFPE tissue samples (formalin-fixed, paraffin-embedded)
    Approx. 2x 5 μm, more starting material may also be used (optionally)

    Average yield
    Depends on the sample and the amount used

    Extraction time
    Lysis: approx. 225 minutes (external)
    InnuPure C16 touch protocol: approx. 38 - 47 minutes

    Average purity
    1,8 - 2,0

    Order information
    845-IPP-5916016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-5916096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-5916480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-PPP-5916016 / 16 reactions, 8 reaction per plate (non-filled) / 99,75 €
    845-PPP-5916096 / 96 reactions, 8 reaction per plate (non-filled) / 399,00 €
    845-PPP-5916480 / 480 reactions, 8 reaction per plate (non-filled) / 1554,00 €

    845-IPS-5916016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-5916096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • PME free-circulating DNA Kit-IPC16

    The PME free-circulating DNA Kit - IPC16 is based on new technology, called: PME – Polymer Mediated Enrichment. The procedure doesn’t start with sample lysis, like common used methods or kits. The first step is capturing of free-circulating DNA with a special polymer. Subsequently the captured free-circulating DNA is dissolved in a special buffer and then the DNA is extracted automatically by using the InnuPure® C16 / C16 touch.
    The extraction process is based on binding of the free-circulating DNA on surface modified magnetic particles. After washing steps the nucleic acid is eluted from the magnetic particles with low RNase-free water and is now ready to use.
    The kit contains a Carrier RNA. Addition of Carrier RNA is recommended if extreme low amount of free-circulating DNA is expected. In This case the addition of Carrier RNA can increase the final yield.

    Specifications

    Starting material
    Serum, plasma, cell culture supernatants or mediums and other cell-free body fluids from 1 ml up to 5 ml
    Urine from 5 ml to 10 ml

    Enrichment procedure
    1 ml protocol / approx: 10 minutes
    5 ml protocol / approx: 30 minutes

    Extraction time
    1 ml protocol / approx: 70 minutes
    5 ml protocol / approx: 80 minutes

    Order information
    845-IPP-6116016 / 16 reactions, 8 reaction per plate (prefilled) / 189,00 €
    845-IPP-6116096 / 96 reactions, 8 reaction per plate (prefilled) / 1039,50 €

    845-IPS-6116016 / 16 reactions, 1 reaction per strip (prefilled) / 220,50 €
    845-IPS-6116096 / 96 reactions, 1 Reaktion per strip (prefilled) / 1155,00 €

  • innuPREP Virus DNA/RNA Kit-IPC16

    The novel innuPREP Virus DNA/RNA Kit-IPC16 is an extraction kit for isolating viral DNA and RNA simultaneously from the same sample. Used in conjunction with the InnuPure C16 touch, the kit is suitable for an exceptionally wide variety of starting materials. The kit contains carrier nucleic acids so that researchers can perform an internal extraction control to prevent false-negative findings (using innuDETECT Internal Control kits). The system automatically processes up to 16 samples in pre-filled Reagent Strips/Plates. In addition to internal lysis, the viral nucleic acids are bound to magnetic and/or paramagnetic particles, washed and then eluted. The sealed Reagent Plastic effectively prevents cross-contamination among samples. Manual prep steps are reduced as well, which minimizes contact between users and infectious materials.
    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Preparation of up to 200 μl, 400 μl and even 600 μl serum, plasma and other cell-free body fluids

    Automation for ideal reproducibility
    Improvement of qPCR results: win up to 1.7 Ct values
    Sensitive protocols for optimized yields

    Starting material
    Serum (200 μl)
    Plasma (200 μl)
    Cell-free bodily fluids (200 μl)
    Cell culture supernatants (200 μl)
    Swab samples
    Stool samples (200 μl)

    Extraction time
    Lysis: internal
    InnuPure C16 touch protocol: approx. 75 minutes
    verage yield
    Depends on the type and quantity of the Starting material

    Average purity
    1,8–2,0

    Order information
    845-IPP-7016016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-7016096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-7016480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-PPP-7116016 / 16 reactions, 8 reaction per plate (non-filled) / 99,75 €
    845-PPP-7116096 / 96 reactions, 8 reaction per plate (non-filled) / 399,00 €
    845-PPP-7116480 / 480 reactions, 8 reaction per plate (non-filled) / 1554,00 €

    845-IPS-7016016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-7016096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • deltaPREP Virus DNA/RNA Kit-IPC16 (MDX)

    CE-IVD Version for diagnostic purposes!

    The novel deltaPREP Virus DNA/RNA Kit-IPC16 is an extraction kit for isolating viral DNA and RNA simultaneously from the same sample. Used in conjunction with the InnuPure C16 touch, the kit is suitable for an exceptionally wide variety of starting materials. The kit contains carrier nucleic acids so that researchers can perform an internal extraction control to prevent false-negative findings (using innuDETECT Internal Control kits). The system automatically processes up to 16 samples in pre-filled Reagent Strips/Plates. In addition to internal lysis, the viral nucleic acids are bound to magnetic and/or paramagnetic particles, washed and then eluted. The sealed Reagent Plastic effectively prevents cross-contamination among samples. Manual prep steps are reduced as well, which minimizes contact between users and infectious materials.

    Specifications

    Preparation of up to 200 μl, 400 μl and even 600 μl serum, plasma and other cell-free body fluids
    Automation for ideal reproducibility
    Improvement of qPCR results: win up to 1.7 Ct values
    Sensitive protocols for optimized yields

    Starting material
    Serum (200 μl)
    Plasma (200 μl)
    Cell-free bodily fluids (200 μl)
    Cell culture supernatants (200 μl)
    Swab samples
    Stool samples (200 μl)

    Extraction time
    Lysis: internal
    InnuPure C16 touch protocol: approx. 75 minutes
    verage yield
    Depends on the type and quantity of the Starting material

    Average purity
    1,8–2,0

    Order information
    31-DP-4010016 / 16 reactions, 8 reaction per plate (prefilled) / 131,25 €
    31-DP-4010096 / 96 reactions, 8 reaction per plate (prefilled) / 635,25 €
    31-DP-4010480 / 480 reactions, 8 reaction per plate (prefilled) / 2572,50 €

    31-DP-5010016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    31-DP-5010096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

  • innuPREP AniPath DNA/RNA Kit-IPC16

    The innuPREP AniPath DNA/RNA Kit – IPC16 is designed for the automated extraction of both bacterial DNA and viral DNA/RNA by using the InnuPure C16 touch. The kit is suitable for a broad spectrum of starting material ranging from cell culture supernatant, cell-free body fluids and whole blood as well as fecal samples, swabs and other relevant material. The kit is based on a patented extraction chemistry and provides best yields of pure nucleic acids accompanied by an accelerated procedure.

    Pre-filled reagent strips or plates reduce required manual steps and thereby minimize the risk of errors. After binding to surface-modified magnetic particles and multiple wash steps, nucleic acids are eluted with RNase-free water. The resulting DNA/RNA can directly be used in subsequent downstream applications such as qPCR. To verify the extraction process the kit contains a Carrier Mix composed of Carrier RNA, Internal Control DNA (IC DNA) and RNA (IC RNA).
    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    One kit for all sample types: broad spectrum of starting material ranging from cell-free body fluids to whole blood and fecal samples
    Pre-filled kit for extraction of bacterial DNA and viral DNA/RNA in parallel
    Automated extraction of up to 16 samples using the InnuPure C16 touch
    Ideally suited for veterinary diagnostics

    Starting material
    Cell culture supernatant, cell-free body fluids (up to 400 µl)
    Whole blood (up to 400 µl)
    Fecal samples (50-100 mg)
    Swabs
    Organ tissue, e.g. spleen, brain, tonsils, lungs, lymph nodes (5-10 mg)

    Average yield
    Depending on sample quality/quantity

    Extraction time
    84 min

    Order information
    845-IPP-8016016 / 16 reactions, 8 reaction per plate (prefilled) / 126,00 €
    845-IPP-8016096 / 96 reactions, 8 reaction per plate (prefilled) / 609,00 €
    845-IPP-8016480 / 480 reactions, 8 reaction per plate (prefilled) / 2467,50 €

    845-PPP-8016016 / 16 reactions, 8 reaction per plate (non-filled) / 99,75 €
    845-PPP-8016096 / 96 reactions, 8 reaction per plate (non-filled) / 399,00 €
    845-PPP-8016480 / 480 reactions, 8 reaction per plate (non-filled) / 1554,00 €

    845-IPS-8016016 / 16 reactions, 1 reaction per strip (prefilled) / 152,25 €
    845-IPS-8016096 / 96 reactions, 1 Reaktion per strip (prefilled) / 750,75 €

Automated Extraction with InnuPure C16touch - SmartExtraction
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  • smart Blood DNA Midi prep (a)

    The smart Blood DNA Midi prep (a)/ smart Blood DNA Midi prep (a96) is based on the unique SmartExtraction technology and allows an easy automation of DNA extraction from up to 3 ml of whole blood. Initially erythrocytes are lysed and PBMCs are collected for further preparation. Pre-filled, sealed Reagent Plastic simplifies handling enormously and can be applied on InnuPure systems directly. Additional tools are not necessary. Possible shearing effects on DNA are reduced to an absolute minimum due to the special SmartExtraction pipette tips, which contain modified surfaces for efficient binding of nucleic acids. Finally high DNA yields (up to 90 μg from 3 ml whole blood) with high molecular weight (up to 500 kbp) can be isolated. An excellent quality ensures the direct application in further downstream analyses.

    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Suitable for all common stabilizers: EDTA, citrate and heparin
    Based on SmartExtraction technology for easy automation without any additional tools needed
    Isolation of high-molecular-weight DNA with a yield up to 90 μg from 3 ml whole blood
    Includes pre-filled Reagent Strips or Plates for simple handling and a minimum number of working steps

    Average Yield
    Depends on sample and used volume
    0.5 ml whole blood: approx. 5 – 15 μg gDNA
    1.0 ml whole blood: approx. 15 – 30 μg gDNA
    2.0 ml whole blood: approx. 40 – 70 μg gDNA
    3.0 ml whole blood: approx. 50 – 90 μg gDNA

    Starting Material
    Whole blood samples of 0.5 to 3 ml
    Fresh or frozen blood
    Stabilizers: EDTA, citrate or heparin
    Extraction Time

    Lysis of erythrocytes: approx. 20 min.
    Automatic processing: approx. 40 to 90 minutes (depending on protocol and device)

    Quality
    1.7 – 2.0

    Order information
    845-ASP-1208016 / 16 reactions, 8 reaction per plate (prefilled) / 131,25 €
    845-ASP-1208096 / 96 reactions, 8 reaction per plate (prefilled) / 630,00 €

    845-ANP-1208016 / 16 reactions, 8 reaction per plate (non-filled) / 94,50 €
    845-ANP-1208096 / 96 reactions, 8 reaction per plate (non-filled) / 430,50 €
    845-ANP-1208480 / 480 reactions, 8 reaction per plate (non-filled) / 1627,50 €

    845-ASS-1208016 / 16 reactions, 1 reaction per strip (prefilled) / 157,50 €
    845-ASS-1208096 / 96 reactions, 1 Reaktion per strip (prefilled) / 756,00 €

  • smart DNA prep (a)

    smart DNA prep (a) is a universal DNA extraction kit, which can be used with the InnuPure systems. Unmatched DNA yields with ideal quality can be automatically isolated from tissue samples, eukaryotic cells or pieces of rodent tails, as well as bacteria and yeast cells. After samples lysis all necessary steps are processed by using one of the pipetting platforms. The usage of Smart Modified Surfaces in combination with optimized pipetting routines efficiently avoids the shearing of DNA and allows for extraction of high-molecular-weight nucleic acids up to 500 kbp. Those can be directly applied to subsequent analysis.
    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Isolation of high-molecular-weight DNA from tissue samples, pieces of rodent tails, eukaryotic cells, bacteria and yeast cells
    Enormous DNA yields and reduced preparation time
    Minimum steps of preparation thanks to pre-filled, sealed Reagent Strips or Reagent Plates

    Starting Material
    Tissue samples up to 100 mg
    Mouse tail pieces up to 1 cm
    Rat tail pieces up to 0.5 cm
    Eukaryotic cells up to 5x 106
    Gram- or Gram+ bacteria (5 x 104 up to 2 x 109 cells)
    Yeast cells 1 x 105 up to 5 x 108

    Extraction Time
    Lysis: depending on starting material
    Automatic processing: approx. 30 to 65 minutes (depending on protocol and device)

    Quality
    1.7 – 2.0

    Average Yield
    Depends on sample and used volume

    Order information
    845-ASP-2008016 / 16 reactions, 8 reaction per plate (prefilled) / 131,25 €
    845-ASP-2008096 / 96 reactions, 8 reaction per plate (prefilled) / 630,00 €

    845-ANP-2008016 / 16 reactions, 8 reaction per plate (non-filled) / 94,50 €
    845-ANP-2008096 / 96 reactions, 8 reaction per plate (non-filled) / 430,50 €
    845-ANP-2008480 / 480 reactions, 8 reaction per plate (non-filled) / 1627,50 €

    845-ASS-2008016 / 16 reactions, 1 reaction per strip (prefilled) / 157,50 €
    845-ASS-2008096 / 96 reactions, 1 Reaktion per strip (prefilled) / 756,00 €

  • smart Blood DNA Midi Direct prep (a)

    smart Blood DNA Midi Direct prep (a) and smart Blood DNA Midi Direct prep (a96) is a kit for extraction of genomic DNA from up to 1 ml whole blood samples. Pre-filled, sealed Reagent Strips or Reagent Plates can be used with InnuPure systems. The innovative 2-step-lysis, consisting of collecting DNA-carrying cells and digestion, as well as all following purification steps are fully automated on the liquid handling platform of choice. The pre-installed routines on the InnuPure C16 systems in combination with the unique SmartExtraction pipette tip allow for isolation of high-molecular-weight DNA with excellent yield and purity. The Smart Modified Surfaces within the 1 ml tip guarantee highest binding capacity and avoid clotting, that often occur when using magnetic particles.
    As an alternative to the standard, pre-filled, sealed extraction kits for InnuPure C16 touch, corresponding lower-cost non-filled kit variants are also available. These kits also contain all the plastics and reagents required for extraction.

    Specifications

    Based on the unique SmartExtraction technology
    Isolation of high-molecular-weight DNA up to 500 kbp
    Efficient 2-step-lysis starts with collection of DNA-carrying cells followed by cell digestion using the extraction automat

    Starting material
    Whole blood samples of 0.2 to 1 ml
    Fresh or frozen blood
    Stabilizers: EDTA or heparin

    Extraction time
    Automatic processing:
    approx. 80 to 165 minutes (depending on protocol and device)

    Average yield
    Depends on sample and used volume
    0.5 ml whole blood: approx. 5 – 15 μg gDNA
    1.0 ml whole blood: approx. 15 – 30 μg gDNA

    Quality
    1.7 – 2.0

    Order information
    845-ASP-3008016 / 16 reactions, 8 reaction per plate (prefilled) / 131,25 €
    845-ASP-3008096 / 96 reactions, 8 reaction per plate (prefilled) / 630,00 €

    845-ANP-3008016 / 16 reactions, 8 reaction per plate (non-filled) / 94,50 €
    845-ANP-3008096 / 96 reactions, 8 reaction per plate (non-filled) / 430,50 €
    845-ANP-3008480 / 480 reactions, 8 reaction per plate (non-filled) / 1627,50 €

    845-ASS-3008016 / 16 reactions, 1 reaction per strip (prefilled) / 157,50 €
    845-ASS-3008096 / 96 reactions, 1 Reaktion per strip (prefilled) / 756,00 €

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