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Automated Extraction / CyBio FeliX

Automated Extraction CyBio FeliX - Magnet particle based
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  • innuPREP Plant DNA Kit-FX

    Using the innuPREP Plant DNA Kit-FX, it is possible to isolate highly pure genomic DNA from various plant materials. After efficient homogenization using SpeedMill PLUS or another homogenizer, or using a mortar and liquid nitrogen, the plant material is lysed and both proteins and polysaccharides are effectively removed in a precipitation step. The lysate is filtered and then transferred to the plates, which are already self-filled or filled using CyBio FeliX. Using magnetic particle separation, nucleic acid extraction is automated on the CyBio FeliX. The final eluate is highly pure, free of magnetic particles and can therefore be used directly in subsequent applications, e.g. qPCR. The kit has already been successfully tested on leaves and herbs, corn, cornmeal and seeds.

    To reduce the manual preparation steps for this kit to a minimum, the innuPREP Prefilling Set (OL3317-25-127 available at Analytik Jena GmbH) is also available for automated prefilling of deep well plates with the appropriate buffers and reagents by CyBio FeliX.

    Specifications

    High throughput application for up to 96 samples in parallel
    Effective removal of inhibitory by-products, such as plant secondary metabolites
    Highly reproducible yields of the DNA to be isolated

    Starting material
    Grain flour or seeds (max. 20-60 mg)
    Sugar beets, leaves or herbs (10-40 mg fresh material / 1-4 mg dried material)

    Extraction time
    Lysis: approx. 40-45 minutes
    CyBio FeliX protocol: approx. 26 minutes

    Average purity
    1,8-2,0

    Order information
    845-FX-2196096 / 96 reactions / 330,75 €
    845-FX-2196480 / 480 reactions / 1207,50 €

  • innuPREP Blood DNA Mini Kit-FX

    The innuPREP Blood DNA Mini Kit-FX has been designed for automated extraction of genomic DNA (gDNA) from whole blood preparations including fresh or frozen whole blood samples stabilized with EDTA, citrate or heparin and even collected in PAXgene® Blood DNA Tubes. The extraction protocol is established on CyBio FeliX based on sample volumes of up to 400 µl. The extraction process is based on binding the nucleic acids to magnetic particles. The procedure starts with the lysis of the starting material. After washing steps the nucleic acid is eluted from the magnetic particles with RNase-free water. The protocol is optimized to obtain DNA of maximum yield and quality which can be used for downstream applications like standard or real-time PCR. The extraction procedure is based on the special technology patented by IST Innuscreen.
    In order to minimize manual preparation steps for this kit, the innuPREP Pre-filling Set (OL3317-25-127 available at Analytik Jena) offers the possibility of automated pre-filling of all Deep Well Plates with respective buffers and reagents by the CyBio FeliX.

    Specifications

    Automated procedure including lysis
    High-throughput application for up to 96 samples in parallel
    Diverse starting material: fresh or frozen whole blood samples stabilized with EDTA, citrate, heparin or collected in PAXgene® Blood DNA Tubes
    Flexible application: protocol for 200 µl and 400 µl
    High yield and high purity DNA

    Starting material
    Up to 400 µl whole blood
    Fresh or frozen blood
    Stabilized with EDTA, citrate or heparin
    PAXgene® Blood DNA Tubes

    Average yield
    Depending on sample quality/amount of mononuclear blood cells
    200 µl whole blood approx. 2 – 8 µg
    400 µl whole blood approx. 2 – 12 µg

    Extraction time
    Up to 200 µl whole blood: 70 min
    Up to 400 µl whole blood: 86 min

    Average purity
    1.8 – 2.0

    Order information
    845-FX-2296096 / 96 reactions / 399,00 €
    845-FX-2296480 / 480 reactions / 1554,00 €

  • innuPREP AniPath DNA/RNA Kit-FX

    The innuPREP AniPath DNA/RNA Kit - FX has been specially developed for the automated extraction of both bacterial DNA and viral DNA or RNA using CyBio FeliX. A wide variety of starting materials can be used such as cell culture supernatants, cell-free body fluids such as plasma and serum, whole blood, stool samples, swabs and other relevant materials. Thanks to the underlying patented extraction chemistry, the nucleic acid purification process achieves best yields of highly pure nucleic acids with significant time savings. After binding of the DNA or RNA to surface-modified magnetic particles and several washing steps, the final elution takes place in RNase-free water. The resulting DNA or RNA is directly available for subsequent applications such as Real Time PCR. For verification purposes, the protocol also includes a carrier mix containing carrier RNA and internal controls.

    To reduce the manual preparation steps for this kit to a minimum, the innuPREP Prefilling Set (OL3317-25-127 available via Analytik Jena GmbH) is also available for automated prefilling of deep well plates with the appropriate buffers and reagents by CyBio FeliX.

    Specifications

    One kit for everything: extensive range of starting materials including cell-free body fluids, whole blood and stool samples
    Simultaneous purification of bacterial DNA and viral DNA or RNA
    High-throughput application for up to 96 samples in parallel
    Ideally suited for use in veterinary diagnostics

    Starting material
    Cell culture supernatant, cell-free body fluids (up to 400 µl)
    Whole blood (up to 400 µl)
    Stool sample (50-100 mg)
    Swab
    Organ tissue, e.g. spleen, brain, tonsils, lungs, lymph nodes (5-10 mg)

    Extraction time
    Lysis: depending on starting material used
    Extraction: 86 min

    Order information
    845-FX-2396096 / 96 reactions / 399,00 €
    845-FX-2396480 / 480 reactions / 1554,00 €

  • innuPREP FFPE DNA Kit-FX

    The combination of the innuPREP FFPE DNA Kit-FX and the CyBio FeliX provides a platform for simple, safe, automated DNA extraction from formalin-fixed, paraffin-embedded (FFPE) tissue samples. Due to a novel chemistry, the otherwise common and time-consuming process for deparaffinization is completely eliminated. This means that genomic DNA can be isolated without the use of toxic solvents such as xylene or octane. In addition, proteinase K digestion is used to degrade proteins in the cell lysates and release the nucleic acids. Based on the patented technology and the principle of magnetic particle separation, DNA purification then follows using InnuPure C16 touch. Prefilled and seethed Reagent Strips or Plates greatly reduce the risk of cross-contamination between samples. The highly pure DNA obtained in this way is directly available for subsequent applications (e.g. qPCR).

    To reduce the manual preparation steps for this kit to a minimum, the innuPREP Prefilling Set (OL3317-25-127 available via Analytik Jena GmbH) is also available for automated prefilling of deep well plates with the appropriate buffers and reagents by CyBio FeliX.

    Specifications

    Without the usual use of the toxic solvents xylene or octane
    High-throughput application for up to 96 samples in parallel

    Starting material
    Paraffin samples (FFPE material: formalin-fixed, paraffin-embedded).
    Up to 2 x 5 μm, optionally more material

    Extraction time
    Lysis: approx. 120 minutes (external)
    CyBio FeliX protocol: approx. 54 minutes

    Average Yield.
    Depending on the sample and the amount used.

    Average purity
    1,8 - 2,0

    Order information
    845-FX-2496096 / 96 reactions / 399,00 €
    845-FX-2496480 / 480 reactions / 1554,00 €

  • innuPREP Food DNA I Kit-FX

    The innuPREP Food DNA I Kit – FX has been designed for the completely automated isolation of DNA from 1 mL of a stomacher cultivation. The extraction procedure is based on a newly patented chemistry. The kit is designed to be handled by educated personnel in a laboratory environment.
    All steps of the extraction process are automated and run completely on the CyBio FeliX. The extraction process starts with an enzymatic lysis of bacterial cells. The extraction is based on binding of DNA to surface-modified magnetic particles. After several washing steps, the nucleic acids are eluted from the magnetic particles with RNase-free Water and are ready to be used for downstream applications. The extraction chemistry in combination with the CyBio FeliX protocol is optimized to get maximum yield and quality.

    To reduce the manual preparation steps for this kit to a minimum, the innuPREP Prefilling Set (OL3317-25-127 available via Analytik Jena GmbH) is also available for automated prefilling of deep well plates with the appropriate buffers and reagents by CyBio FeliX.

    Specifications

    High-throughput application for up to 96 samples in parallel

    Starting material
    1 mL culture from a stomacher cultivation

    Extraction Time
    108 min with pre-lysis;
    75 min without pre-lysis

    Average purity
    1,8 - 2,0

    Order information
    845-FX-2696096 / 96 reactions / 330,75 €
    845-FX-2696480 / 480 reactions / 1207,50 €

  • innuPREP Virus TS RNA Kit 2.0-FX

    IST Innuscreen has designed a new extraction kit to meet the current enormous increase in demand for extraction kits and the associated global shortage of resources. The innuPREP Virus TS RNA Kit 2.0-FX was specially developed for isolating Sars-CoV-2 RNA from respiratory swab samples such as nasopharyngeal and oropharyngeal swabs using the CyBio FeliX in combination with the CyBio FeliX Extraction Set. Up to 96 samples can be processed automatically. The novel simplified kit chemistry allows an efficient extraction process based on the binding of RNA to surface-modified magnetic particles. After washing, the RNA is eluted with RNase-free water and the nucleic acid is available for subsequent applications. In addition, the kit contains carrier RNA, which optimizes the yield, especially in case of small amounts of RNA samples. In addition, this RNA also contains an internal control RNA, which can be used as an extraction control. Please note that ethanol and 2-propanol must be provided for the extraction.

    In order to minimize manual preparation steps for this kit, the innuPREP Pre-filling Set (OL3317-25-127 available at Analytik Jena) offers the possibility of automated pre-filling of all Deep Well Plates with respective buffers and reagents by the CyBio FeliX.

    The innuPREP Virus TS RNA Kit 2.0-FX is intended for research use only.
    A special package of this extraction kit is also available for applications with maximum sample throughput.

    Specifications

    Automated routine using the CyBio FeliX
    Allows processing of up to 96 samples
    Includes carrier RNA for maximum yields

    Starting material
    Swab samples
    Nasopharyngeal swabs samples
    Oropharyngeal swabs samples

    Average Yield
    Depending on sample quality/viral load

    Extraction time
    62 min

    Order information
    845-FX-2796096 / 96 reactions / 294,00 €
    845-FX-27969600 / 9600 reactions / 23520,00 €

  • innuPREP Virus DNA/RNA Kit-FX

    The innuPREP Virus DNA/RNA Kit-FX has been designed for automated and simultaneous extraction of both viral DNA and RNA from serum or plasma samples. The extraction protocol is established on CyBio FeliX based on sample volumes of 200 µl or 400 µl. Based on the reliable magnetic separation upon nucleic acid binding to magnetic particles up to 96 samples can be processed in parallel. Moreover, the extraction procedure is based on a special technology patented by IST Innuscreen. The procedure starts with the lysis of the starting material. After washing steps the nucleic acid is eluted from the magnetic particles with RNase-free water. Resulting viral DNA and RNA are maximal in yield and quality and can be used for downstream applications like standard or real-time PCR. To verify the extraction process, the kit contains a Carrier Mix composed of Carrier RNA, Internal Control DNA (IC DNA) and RNA (IC RNA).
    In order to minimize manual preparation steps for this kit, the innuPREP Pre-filling Set (OL3317-25-127 available at Analytik Jena) offers the possibility of automated pre-filling of all Deep Well Plates with respective buffers and reagents by the CyBio FeliX.

    Specifications

    Automated application based on CyBio Felix
    Nucleic acid extraction from up to 96 samples
    Optimized for serum and plasma
    Flexible application: protocol for 200 µl and 400 µl sample volumes
    Best yields of high purity DNA and RNA

    Starting material
    Up to 400 µl sample volume
    Serum
    Plasma

    Average Yield
    Depending on sample quality/quantity

    Extraction time
    Up to 200 µl: 62 min
    Up to 400 µl: 73 min

    Average purity
    1.8 – 2.0

    Order information
    845-FX-2096096 / 96 reactions / 399,00 €
    845-FX-2096480 / 480 reactions / 1554,00 €

Automated Extraction CyBio FeliX - SmartExtraction
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  • smart DNA prep (a96)-FX

    The universal smart DNA prep (a96) – FX Kit has been designed for automated isolation of high-molecular-weight genomic DNA from a broad variety of starting materials including tissue samples, eukaryotic cells, rodent tails, bacteria and yeast cells using CyBio FeliX. The extraction is based on the new SmartExtraction technology (patent pending). The whole extraction process just needs simple pipetting up and down and profits from efficient binding of DNA to Smart Modified Surfaces inside the filter tip. After washing, the ultra-pure DNA is eluted and ready for use in subsequent downstream applications.
    The innuPREP Bacteria Lysis Booster is also available to further optimize the lysis of bacterial cells, particularly with regard to bacterial strains that are difficult to lyse.

    If needed the customer has the opportunity to use the premium pre-filled kit version in order to reduce hands-on time even more. Alternatively, cost-efficient non-filled kit versions are available, too.

    Specifications

    Optimized for automatic extraction by CyBio FeliX
    Magnificent binding capacity without particle agglutination thanks to unique SmartExtraction technology
    Up to 500 µl elution volume
    Pre-filled reagents minimize preparation steps and save time

    Starting Material
    Tissue sample (1 – 100 mg)
    For tissue samples with a high DNA content (e.g. spleen samples, pancreatic samples and lymph nodes) use max. 5 mg!
    Mouse tails (up to 1.0 cm)
    Rat tails ( up to 0.5 cm)
    Eukaryotic cells (max. 5 x 10exp6)
    Bacteria cell pellets (1 x 10exp5 – 1 x 10exp9 cells)
    Yeast cell pellets (1 x 10exp5 – 1 x 10exp9 cells)

    Average Yield
    Depending on sample quality/amount of starting material

    Extraction Time
    External Lysis: depends on starting material
    Extraction: 40 min

    Order information
    845-FX-4096096 / 96 reactions / 336,00 €
    845-FX-4096480 / 480 reactions / 1344,00 €

    845-PFX-4096096 / 96 reactions, reagents pre-filled / 535,50 €

  • smart Blood Midi prep (a96)-FX

    The smart Blood DNA Midi prep (a96) – FX kit has been designed for automated isolation of high-molecular-weight genomic DNA from peripheral blood mononuclear cells (PBMC) derived from fresh or frozen blood using CyBio FeliX. The kit can handle sample volumes of up to 3 ml, whereby blood samples can be stabilized with EDTA, citrate, heparin as well as with PAXgene® Blood DNA Tubes. The extraction method combines the innovative SmartExtraction technology developed by IST Innuscreen (patent pending). After erythrocyte lysis the PBMCs are pelleted. Subsequently, PBS is added and the cells are resuspended and transferred into the kit’s Reagent Plate. The whole extraction process just needs simple pipetting up and down and profits from efficient binding of genomic DNA to Smart Modified Surfaces inside the filter tip, which minimizes DNA fragmentation. After washing steps, the high molecular weight DNA (up to 350 kbp) is eluted and ready for use in subsequent downstream applications.

    By choosing the premium pre-filled kit version, hands-on time is minimized and handling vastly simplified. However, lower-priced non-filled kit versions are also available.

    Specifications

    Up to 90 µg DNA from of up to 3 ml whole blood
    Excellent performance tested for extraction of DNA from fresh or frozen blood stabilized with EDTA, citrate and heparin, as well as blood sampled with PAXgene® Blood DNA Tube
    High yield of large DNA fragments based on innovative SmartExtraction technology
    Minimal preparation time thanks to pre-filled reagents

    Starting Material
    0.5 – 3 ml whole blood
    Fresh or frozen
    stabilized with EDTA, citrate or heparin
    PAXgene® Blood DNA Tube

    Average Yield
    Depending on sample quality/amount of starting material
    0.5 ml whole blood: 5 – 15 µg
    1.0 ml whole blood: 15 – 30 µg
    2.0 ml whole blood: 40 – 70 µg
    3.0 ml whole blood: 50 – 90 µg

    Extraction Time
    External Lysis: approx. 40 min
    Extraction: 40 min

    Order information
    845-FX-4196096 / 96 reactions / 336,00 €
    845-FX-4196480 / 480 reactions / 1344,00 €

    845-PFX-4196096 / 96 reactions, reagents pre-filled / 535,50 €

  • smart Blood Midi Direct prep (a96)-FX

    The smart Blood DNA Midi Direct prep (a96) – FX kit has been designed for direct and automated isolation of high-molecular-weight genomic DNA from peripheral blood mononuclear cells (PBMC) derived from fresh or frozen blood using CyBio FeliX. Thanks to the innovative internal lysis up to 1 ml whole blood stabilized with EDTA, citrate, heparin as well as sampled with PAXgene® Blood DNA Tubes and buffy coat can be used directly. The use of premium pre-filled kit versions allows an additional saving of time, but low-priced non-filled versions are available as well. After surface adsorption of PBMC, cells are lysed and released genomic DNA efficiently binds to Smart Modified Surfaces exclusively developed by IST Innuscreen (patent pending). Based on the gentle nature of the extraction process DNA fragmentation is minimized. After washing, the high-molecular-weight genomic DNA is eluted and is ready for use in subsequent downstream applications.

    If needed the customer has the opportunity to use the premium pre-filled kit version in order to reduce hands-on time even more. Alternatively, cost-efficient non-filled kit versions are available, too.

    Specifications

    Automatic high-throughput application including lysis
    Excellent performance tested for extraction of DNA from fresh or frozen blood stabilized with EDTA, citrate and heparin, PAXgene® Blood DNA Tube or buffy coat
    Minimal hands-on time thanks to pre-filled kit version
    High yield and quality of extracted DNA

    Starting Material
    0.2 – 1.0 ml whole blood
    Fresh or frozen blood (max. two freeze/thaw cycles)
    stabilized with EDTA, citrate, heparin
    PAXgene® Blood DNA Tube
    0.2 – 1.0 ml buffy coat (derived from up to 2.0 ml stabilized whole blood)1
    1 The blood has to fulfill the conditions described for extraction from whole blood.

    Average Yield
    Depending on sample quality/amount of starting material
    Up to 500 µl: 5 – 15 µg
    Up to 1,000 µl: 15 – 40 µg
    Using buffy coat of an equivalent of 2 ml whole blood: 30 – 60 µg

    Extraction Time
    Depending on sample volume
    Up to 500 µl: 90 minutes
    Up to 1,000 µl: 120 minutes

    Order information
    845-FX-4296096 / 96 reactions / 336,00 €
    845-FX-4296480 / 480 reactions / 1344,00 €

    845-PFX-4296096 / 96 reactions, reagents pre-filled / 577,50 €

  • smart Plant DNA prep (a96)-FX

    The smart Plant DNA prep (a96) – FX has been designed for automated isolation of high-molecular-weight genomic DNA (gDNA) from plant material using the liquid handling platform CyBio FeliX. After the homogenization of dried or fresh plant parts including leafs, roots, seeds and more, DNA is bound to Smart Modified Surfaces, which were exclusively developed by IST Innuscreen (patent pending). The easy procedure predominantly based on simple pipetting up and down results in high yields of extracted DNA.

    If needed the customer has the opportunity to use the premium pre-filled kit version in order to reduce hands-on time even more. Alternatively, cost-efficient non-filled kit versions are available, too.

    Specifications

    Automatic extraction from up to 96 samples in parallel
    Excellent performance tested for extraction of DNA from fresh and dried plant material
    Elimination of interfering plant metabolites and possible PCR inhibitors
    Cost-efficient non-filled and premium pre-filled kit version available

    Starting Material
    Disrupted plant material e.g. with SpeedMill PLUS
    20 – 60 mg cornmeal
    20 – 60 mg seeds
    10 - 40 mg sugar beet (fresh)
    1 - 4 mg corn leaf (dried)

    Average Yield
    Depending on amount and condition of starting material

    Extraction Time
    Lysis: Depends on starting material used
    Extraction: 40 min

    Order information
    845-FX-4396096 / 96 Reactions / 294,00 €
    845-FX-4396480 / 480 Reactions / 1207,50 €

    845-PFX-4396096 / 96 reactions, reagents pre-filled / 525,00 €

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